Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation
1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant tr...
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| Veröffentlicht in: | Comparative biochemistry and physiology. B, Comparative biochemistry Jg. 96; H. 2; S. 349 |
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| Format: | Journal Article |
| Sprache: | Englisch |
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England
1990
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| ISSN: | 0305-0491 |
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| Abstract | 1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells. |
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| AbstractList | 1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells. 1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells.1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells. |
| Author | Mitani, H Hayasaka, K Sato, M Shima, A |
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| Snippet | 1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in... |
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| SubjectTerms | Animals Anti-Bacterial Agents Cell Division Cell Line Cell Transformation, Neoplastic Cells, Cultured Drug Resistance - genetics Genes, ras Gentamicins Goldfish Humans Mice Transfection Urinary Bladder Neoplasms |
| Title | Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation |
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