Shrimp Plasma MANF Works as an Invertebrate Anti-Inflammatory Factor via a Conserved Receptor Tyrosine Phosphatase

For a long time, how anti-inflammatory factors evolved was largely unknown. In this study, we chose a marine invertebrate, , as a model and identified that shrimp mesencephalic astrocyte-derived neurotrophic factor (MANF) was an LPS-induced plasma protein, which exerted its anti-inflammatory roles o...

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Veröffentlicht in:The Journal of immunology (1950) Jg. 208; H. 5; S. 1214
Hauptverfasser: Luo, Kaiwen, Chen, Yaohui, Wang, Fan
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.03.2022
Schlagworte:
Amino Acid Sequence
Animals
Apoptosis - immunology
Cell Line
Endoplasmic Reticulum Stress - physiology
Extracellular Signal-Regulated MAP Kinases - metabolism
HEK293 Cells
Humans
Inflammation - pathology
Lipopolysaccharides - metabolism
MAP Kinase Signaling System - physiology
Nerve Growth Factors - metabolism
Neurons - physiology
Penaeidae - immunology
Phosphorylation - physiology
Receptor Protein-Tyrosine Kinases - metabolism
Sequence Alignment
ISSN:1550-6606, 1550-6606
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Zusammenfassung:For a long time, how anti-inflammatory factors evolved was largely unknown. In this study, we chose a marine invertebrate, , as a model and identified that shrimp mesencephalic astrocyte-derived neurotrophic factor (MANF) was an LPS-induced plasma protein, which exerted its anti-inflammatory roles on shrimp hemocytes by suppressing ERK phosphorylation and Dorsal expression. In addition, we demonstrated that shrimp MANF could be associated with a receptor protein tyrosine phosphatase (RPTP) to mediate negative regulation of ERK activation and Dorsal expression. More interestingly, shrimp RPTP-S overexpression in 293T cells could switch shrimp and human MANF-mediated ERK pathway activation to inhibition. In general, our results indicate that this conserved RPTP is the key component for extracellular MANF-mediated ERK pathway inhibition, which gives a possible explanation about why this neurotropic factor could both protect neuron cells from apoptosis and inhibit immune cell M1 activation in various species.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1550-6606
1550-6606
DOI:10.4049/jimmunol.2100595