Nonclassical behavior of the mouse CD1 class I-like molecule

The mouse CD1 (mCD1) molecule is a class I-like molecule that is encoded outside of the MHC. We show here that mCD1 shares several properties with Ag-presenting class I molecules, including a requirement for beta2-microglobulin for stable cell-surface expression in T lymphocyte transfectants and thy...

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Veröffentlicht in:	The Journal of immunology (1950) Jg. 158; H. 5; S. 2143
Hauptverfasser:	Teitell, M, Holcombe, H R, Brossay, L, Hagenbaugh, A, Jackson, M J, Pond, L, Balk, S P, Terhorst, C, Peterson, P A, Kronenberg, M
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 01.03.1997
Schlagworte:	Animals Antigens, CD1 - biosynthesis Antigens, CD1 - genetics Antigens, CD1 - immunology ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette, Sub-Family B, Member 3 beta 2-Microglobulin - biosynthesis CD8 Antigens - metabolism Drosophila melanogaster - genetics Histocompatibility Antigens Class I - immunology Lymphoma, T-Cell Mice Protein Binding - immunology T-Lymphocytes - metabolism Thymus Gland - cytology Thymus Gland - metabolism Transfection - immunology Tumor Cells, Cultured
ISSN:	0022-1767
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Zusammenfassung:	The mouse CD1 (mCD1) molecule is a class I-like molecule that is encoded outside of the MHC. We show here that mCD1 shares several properties with Ag-presenting class I molecules, including a requirement for beta2-microglobulin for stable cell-surface expression in T lymphocyte transfectants and thymocytes. mCD1 is also capable of binding to mouse CD8alphabeta heterodimers participating in the activation of CD8+ T cells in a manner similar to classical class I molecules. However, mCD1 surface expression is not decreased at high temperatures in cells that lack the transporter associated with Ag processing (TAP), including both RMA-S and Drosophila melanogaster cells. The data indicate that mCD1 does not require TAP to be expressed in a stable fashion at the cell surface. We speculate that the ability of mCD1 to reach the cell surface in transporter-deficient cells may reflect its ability to present a distinct set of ligands. The properties of mCD1 described here can account, in part, for the selection of the diverse populations of T cells that are known to be mCD1 reactive.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-1767
DOI:	10.4049/jimmunol.158.5.2143