A nanozyme-linked immunosorbent assay for dual-modal colorimetric and ratiometric fluorescent detection of cardiac troponin I

•A new kind of robust nanozyme-linked immunosorbent assay has been developed.•The proposed nanozyme-linked immunosorbent assay can achieve ratiometric fluorescent and colorimetric dual-modal detection.•This presented method affords robust, reliable and sensitive immunoassay performance. To develop a...

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Published in:Sensors and actuators. B, Chemical Vol. 288; pp. 60 - 64
Main Authors: Miao, Luyang, Jiao, Lei, Tang, Qiaorong, Li, He, Zhang, Lianhua, Wei, Qin
Format: Journal Article
Language:English
Published: Lausanne Elsevier B.V 01.06.2019
Elsevier Science Ltd
Subjects:
Biomarkers
Carbon nitride
Colorimetric detecion
Colorimetry
Dual-modal detection
Enzyme-linked immunosorbent assay
Fluorescence
Hydrogen bonding
Hydrogen peroxide
Immunoassay
Line spectra
Peroxidase
Phenylenediamine
Protein quantification
Quantum dots
Ratiometric fluorescence
Robustness
Substrates
Ratiometric fluorescence
Colorimetric detecion
Protein quantification
Enzyme-linked immunosorbent assay
Dual-modal detection
ISSN:0925-4005, 1873-3077
Online Access:Get full text
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Summary:•A new kind of robust nanozyme-linked immunosorbent assay has been developed.•The proposed nanozyme-linked immunosorbent assay can achieve ratiometric fluorescent and colorimetric dual-modal detection.•This presented method affords robust, reliable and sensitive immunoassay performance. To develop a robust method for the detection of disease biomarkers is always the pursued goal in the field of biomedical research. Multi-modal immunoassay strategy will bring tremendous potential for the purpose. In this study, a kind of robust nanozyme-linked immunosorbent assay has been presented, which can achieve ratiometric fluorescent and colorimetric dual-modal sensing performance. Here, o-phenylenediamine (OPD) as an organic substrate can be oxidized and converted into 2,3-diaminophenazine (oxOPD) by the robust nanozyme of nanoceria with peroxidase-like properties in the present of H2O2. An exogenous fluorescence signal source of graphitic carbon nitride quantum dots (g-C3N4 QDs) is introduced, and the corresponding fluorescence can be efficiently quenched by oxOPD owing to the hydrogen bonding and π-π stacking interactions, yielding a fluorescence spectral line with dual emission peaks while only irradiated by an excitation wavelength. Meanwhile, a visible color change was derived from the oxOPD formation, which can be recorded by colorimetric method. As a proof-of-study demonstration, the detection of human cardiac troponin I was done. This method affords robust, reliable and sensitive detection performance due to the combination of the merits of ratiometric fluorescent assay and colorimetric assay. It is no doubt that our proposed approach will bring remarkable inspirations for biomedical researchers.
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ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2019.02.111