Interleukin-1β and tumor necrosis factor-α production by human monocytes cultured with L-thyroxine and thyrocalcitonin: Relation to severe root shortening

The objectives of this study were to determine whether L-thyroxine (T4) and thyrocalcitonin (TCA) influence monocyte production of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) and to examine IL-1βand TNF-αproduction in monocytes from a group of orthodontic patients with severe root short...

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Vydáno v:American journal of orthodontics and dentofacial orthopedics Ročník 110; číslo 4; s. 399 - 404
Hlavní autoři: Rossi, Mario, Whitcomb, Steven, Lindemann, Robert
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Mosby, Inc 01.10.1996
Témata:
Adult
Calcitonin - pharmacology
Case-Control Studies
Cells, Cultured
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Humans
Interleukin-1 - biosynthesis
Lipopolysaccharides - pharmacology
Male
Monocytes - drug effects
Monocytes - metabolism
Root Resorption - diagnosis
Root Resorption - metabolism
Stimulation, Chemical
Thyroxine - pharmacology
Tumor Necrosis Factor-alpha - biosynthesis
ISSN:0889-5406
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Shrnutí:The objectives of this study were to determine whether L-thyroxine (T4) and thyrocalcitonin (TCA) influence monocyte production of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) and to examine IL-1βand TNF-αproduction in monocytes from a group of orthodontic patients with severe root shortening. Human monocytes were incubated with varying concentrations of T4 and TCA for 24 hours, and IL-1βand TNF-αlevels were measured by ELISA. At a concentration of 0.1 μg/ml, T4 and TCA induced significantly more IL-1βthan untreated controls, and T4 induced more IL-1βthan TCA. Neither hormone induced significant TNF-αrelease, conversely, TCA had an inhibitory effect on unstimulated monocyte release of TNF-α. TCA was also shown to inhibit, but not reverse, the activational effect of lipopolysaccharide on monocyte TNF-αrelease. T4 and TCA concentrations as low as 0.1 pg/ml caused monocytes to release significant amounts of IL-1β. The highest concentration of T4 tested (1.0 wg/ml) induced significantly less IL-1βproduction than lower concentrations. T4-αand TCA-treated monocytes bound more labeled IL-1βthan untreated controls, which suggests that these hormones increase IL-1 receptor expression. There was a wide range of unstimulated and stimulated IL-1βand TNF-αproduction by root resorption subject monocytes with no significant differences between resorption and nonresorption group means. This data suggest that patient monocytes did not differ from control monocytes in regard to these cytokine parameters, and therefore in vitro IL-1βand TNF-αlevels could not distinguish resorption subjects. (Am J Orthod Dentofac Orthop 1996;110:399-404.)
Bibliografie:ObjectType-Article-1
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ISSN:0889-5406
DOI:10.1016/S0889-5406(96)70042-2