Differences in the induction of CD8+ T cell responses by subpopulations of dendritic cells from afferent lymph are related to IL-1α secretion

The major subset of dendritic cells (DC) from bovine afferent lymph expresses the SIRPα MyD‐1 antigen, but not CD11a or the antigen recognized by mAb CC81, and potently stimulates CD4+ and CD8+ T lymphocyte proliferation. The minor subpopulation, that is CD11a+CC81+MyD‐1−, effectively stimulates CD4...

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Bibliographic Details
Published in:Journal of leukocyte biology Vol. 69; no. 2; pp. 271 - 279
Main Authors: Hope, Jayne C., Sopp, Paul, Collins, Robert A, Howard, Chris J.
Format: Journal Article
Language:English
Published: United States Society for Leukocyte Biology 01.02.2001
Subjects:
Adjuvants, Immunologic - genetics
Adjuvants, Immunologic - pharmacology
afferent lymph veiled cells
Animals
Antibodies, Monoclonal - analysis
Cattle
CD11 Antigens - biosynthesis
CD11 Antigens - immunology
CD8+ lymphocytes
CD8-Positive T-Lymphocytes - cytology
CD8-Positive T-Lymphocytes - immunology
Cell Separation
Cell Survival - immunology
Cell-Free System - immunology
Cells, Cultured
Clonal Anergy
Cytotoxicity, Immunologic
Dendritic Cells - immunology
Dendritic Cells - metabolism
Down-Regulation - immunology
Humans
Immunophenotyping
Interleukin-1 - genetics
Interleukin-1 - pharmacology
Interleukin-1 - secretion
Interleukin-12 - genetics
Interleukin-12 - pharmacology
Interleukin-2 - secretion
Interleukin-5 - secretion
Interleukin-6 - genetics
Interleukin-6 - pharmacology
Isoantigens - immunology
Lymph - cytology
Lymph - immunology
Lymphocyte Activation
Recombinant Proteins - immunology
Recombinant Proteins - pharmacology
ISSN:0741-5400, 1938-3673
Online Access:Get full text
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Summary:The major subset of dendritic cells (DC) from bovine afferent lymph expresses the SIRPα MyD‐1 antigen, but not CD11a or the antigen recognized by mAb CC81, and potently stimulates CD4+ and CD8+ T lymphocyte proliferation. The minor subpopulation, that is CD11a+CC81+MyD‐1−, effectively stimulates CD4+ but not CD8+ T lymphocyte proliferation. CD11a+CC81+MyD‐1− DC did not induce anergy or death or secrete an inhibitory factor. However, supernatant from cultures of CD8+ T cells with CD11a−CC81−MyD‐1+ DC significantly enhanced proliferation of CD8+ T cells in response to CD11a+CC81+MyD‐1− DC, an effect that was blocked by interleukin (IL)‐1α, but not IL‐1β, specific mAb. The proliferation of CD8+ T cells with CD11a+CC81+MyD‐1− DC was also enhanced by adding IL‐1α. IL‐1β slightly enhanced proliferation, whereas IL‐2, IL‐6, IL‐12, and IL‐15 had no effect. We conclude that the failure to stimulate CD8+ T cell proliferation results from the lack of IL‐1α synthesis by this population, which may have important consequences in vivo.
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ISSN:0741-5400
1938-3673
DOI:10.1189/jlb.69.2.271