Presence of glycosaminoglycans in retinal capillary basement membrane

Retinal microvessels were isolated from bovine eyes and the basement membranes were purified either directly or after incubation with [35S]sulfate and [14C]glucosamine. The basement membranes, which were purified by osmotic lysis and sequential treatment with detergents, had the general compositiona...

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Veröffentlicht in:Biochimica et biophysica acta Jg. 674; H. 3; S. 400
Hauptverfasser: Cohen, M P, Ciborowski, C J
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands 18.05.1981
Schlagworte:
Amino Acids - analysis
Animals
Basement Membrane - analysis
Capillaries - analysis
Carbon Radioisotopes
Cattle
Glycosaminoglycans - analysis
Retina - blood supply
Sulfur Radioisotopes
ISSN:0006-3002
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Zusammenfassung:Retinal microvessels were isolated from bovine eyes and the basement membranes were purified either directly or after incubation with [35S]sulfate and [14C]glucosamine. The basement membranes, which were purified by osmotic lysis and sequential treatment with detergents, had the general compositional features associated with basement membrane collagens, including high levels of hydroxyproline and hydroxylysine and the presence of 3-hydroxyproline and cystine. After pronase digestion, cellulose acetate electrophoresis of glycosaminoglycans from retinal microvessel basement membrane revealed material comigrating with heparan sulfate that was insensitive to digestion with Streptomyces hyaluronidase ad chondroitinase ABC. Retinal microvessels also incorporated [35S]- and [14C]glucosamine into glycosaminoglycans that were isolated following pronase digestion of the retinal microvessel basement membrane purified from these incubations. The findings provide the first demonstration that glycosaminoglycans are integral components of the retinal microvascular basement membrane and suggest that heparan sulfate is the major glycosaminoglycan species in this basement membrane.
Bibliographie:ObjectType-Article-1
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ISSN:0006-3002
DOI:10.1016/0304-4165(81)90370-6