Shining light on drug discovery: optogenetic screening for TopBP1 biomolecular condensate inhibitors

Abstract Human topoisomerase IIβ binding protein 1 (TopBP1) is a scaffold protein involved in DNA replication initiation, DNA repair, transcription regulation, and checkpoint activation. TopBP1 forms nuclear condensates that act as a molecular switch to amplify ATR activity and promote the activatio...

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Bibliographic Details
Published in:	NAR cancer Vol. 7; no. 4; p. zcaf041
Main Authors:	Morano, Laura, Vie, Nadia, Aissanou, Adam, Hodroj, Dana, Garambois, Véronique, Fauvre, Alexandra, Promonet, Alexy, Egger, Tom, Bordignon, Benoît, Hassen-Khodja, Cédric, Fiachetti, Solène, Basbous, Jihane, Gongora, Céline, Constantinou, Angelos
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01.12.2025
Subjects:	5-Fluorouracil Animals Antineoplastic Agents - pharmacology Ataxia Telangiectasia Mutated Proteins - antagonists & inhibitors Ataxia Telangiectasia Mutated Proteins - metabolism Carrier Proteins - antagonists & inhibitors Carrier Proteins - genetics Carrier Proteins - metabolism Cell Line, Tumor Checkpoint Kinase 1 - metabolism Chemotherapy CHK1 protein Chromatin Clinical trials Colorectal carcinoma Condensates DNA biosynthesis DNA repair DNA-Binding Proteins - antagonists & inhibitors DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Drug Discovery - methods Drug resistance Fluorouracil - administration & dosage Fluorouracil - pharmacology Gene regulation High-throughput screening High-Throughput Screening Assays - methods Humans Irinotecan Irinotecan - pharmacology Kinases Mice Nuclear Proteins - antagonists & inhibitors Nuclear Proteins - genetics Nuclear Proteins - metabolism Quinacrine Replication initiation Signal Transduction - drug effects Thimerosal Toxicity Transcription activation Transcription initiation
ISSN:	2632-8674, 2632-8674
Online Access:	Get full text
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