Immunophenotyping of cerebrospinal fluid cells in ischaemic stroke

Background and purpose Post‐ischaemic immune cell invasion into the brain is well characterized in animal stroke models and contributes to neuronal damage. Therefore, it represents a promising therapeutic target. Cerebrospinal fluid (CSF) is easily accessible and may reflect cellular events within t...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:European journal of neurology Ročník 26; číslo 6; s. 919 - 926
Hlavní autoři: Schulte‐Mecklenbeck, A., Kleffner, I., Beuker, C., Wirth, T., Hartwig, M., Schmidt‐Pogoda, A., Klotz, L., Hansen, W., Wiendl, H., Meuth, S. G., Gross, C. C., Minnerup, J.
Médium: Journal Article
Jazyk:angličtina
Vydáno: England John Wiley & Sons, Inc 01.06.2019
Témata:
Animal models
Biomarkers
Brain
Cerebrospinal fluid
flow cytometry
Immune system
immunology
inflammation
ischaemic stroke
Leukocytes
Leukocytes (granulocytic)
Localization
Lymphocytes
Monocytes
Neuroimaging
Parenchyma
Peripheral blood
Proteins
Stroke
Therapeutic applications
ischaemic stroke
flow cytometry
inflammation
immunology
cerebrospinal fluid
ISSN:1351-5101, 1468-1331, 1468-1331
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:Background and purpose Post‐ischaemic immune cell invasion into the brain is well characterized in animal stroke models and contributes to neuronal damage. Therefore, it represents a promising therapeutic target. Cerebrospinal fluid (CSF) is easily accessible and may reflect cellular events within the parenchyma. However, comprehensive studies on CSF immune cells in patients with stroke are lacking. Methods In a retrospective cohort study, we performed extensive immune‐cell profiling in CSF and peripheral blood of patients with acute ischaemic stroke and healthy controls. In patients with stroke, infarct size was quantified on follow‐up imaging. Results Overall, 90 patients with ischaemic stroke and 22 controls were included in our study. After stroke, the total protein was increased (537.3 vs. 353.2 mg/L, P = 0.008) and the mean total white cell count was slightly but non‐significantly elevated (1.76 vs. 0.50 cells/μL, P = 0.059). Proportions of CSF lymphocytes, monocytes and granulocytes and their respective subsets did not differ between patients with stroke and controls. In addition, there were no associations between proportions of major leukocyte subsets in CSF and the time from symptom onset to CSF sampling, infarct size or infarct localization. Conclusions Ischaemic stroke induces only a very slight increase of CSF immune cells without changes in the composition of immune cell subsets, thus indicating that parenchymal inflammation is not sufficiently reflected in the CSF. Our findings suggest that CSF is not a major invasion route for immune cells and that CSF cell analyses are not suitable as biomarkers to guide future immune therapies for stroke.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1351-5101
1468-1331
1468-1331
DOI:10.1111/ene.13909