Bone marrow mesenchymal stem cells regulate TGF‐β to adjust neuroinflammation in postoperative central inflammatory mice

Background Postoperative cognitive dysfunction (POCD) is one of the common postoperative complications, which is more common in aged patients. POCD mainly manifests as cognitive function changes after surgery, such as memory decline and inattention. In some severe cases, patients may suffer from per...

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Vydané v:Journal of cellular biochemistry Ročník 121; číslo 1; s. 371 - 384
Hlavní autori: Sun, Zhen‐Zhen, Li, Yun‐Feng, Xv, Zhi‐Peng, Zhang, You‐Zhi, Mi, Wei‐Dong
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States Wiley Subscription Services, Inc 01.01.2020
Predmet:
Animal models
Animals
Apoptosis
Behavior, Animal
BMSCs
Bone marrow
Bone Marrow Cells - metabolism
Cell Differentiation - drug effects
Cell Survival
Cognitive ability
Cognitive Dysfunction - metabolism
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Growth factors
Inflammation
Inflammation - metabolism
Inhibition (psychology)
Lipopolysaccharides
Lipopolysaccharides - metabolism
Male
Mesenchymal Stem Cell Transplantation
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchyme
Mice
Mice, Inbred C57BL
neuroinflammation
Neurons - metabolism
postoperative central inflammatory
Postoperative Period
Protein expression
Proteins
Signal Transduction
Signaling
Smad2 protein
Smad2 Protein - metabolism
Social behavior
Stem cell transplantation
Stem cells
Surgery
TGF‐β
Transforming Growth Factor beta - metabolism
Transforming growth factor-b
TGF-β
postoperative central inflammatory
neuroinflammation
BMSCs
ISSN:0730-2312, 1097-4644, 1097-4644
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Shrnutí:Background Postoperative cognitive dysfunction (POCD) is one of the common postoperative complications, which is more common in aged patients. POCD mainly manifests as cognitive function changes after surgery, such as memory decline and inattention. In some severe cases, patients may suffer from personality changes and (or) social behavior decline. The aim of the current study is to confirm the effect and elucidate the mechanism of bone marrow mesenchymal stem cells (BMSCs) in postoperative central inflammatory mice. Methods Mice were randomly assigned to four groups: sham, sham+BMSCs, model, and BMSCs group. In the model group, mice were intraperitoneally injected 8 mg/kg per day lipopolysaccharide for 5 days. In sham+BMSCs and BMSCs group, BMSCs (1 × 10 7) in 100 µL saline were injected into sham mice and model mice, respectively. Results In the model group, transforming growth factor β (TGF‐β) protein expression was significantly increased, compared with that in the sham group. BMSCs were treated into postoperative central inflammatory mice, which resulted in a decreased of TGF‐β protein expression. TGF‐β and smad2 protein expression were suppressed, and apoptosis rate and inflammation were inhibited in coculture with BMSCs. The suppression of TGF‐β inhibited the effects of BMSCs on apoptosis rate and inflammation in postoperative central inflammatory through a smad2 signaling pathway. The promotion of TGF‐β reduced the effects of BMSCs on apoptosis rate and inflammation in postoperative central inflammatory through a smad2 signaling pathway. Conclusion The present study demonstrates that BMSCs regulates TGF‐β to adjust neuroinflammation in postoperative central inflammatory mice.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0730-2312
1097-4644
1097-4644
DOI:10.1002/jcb.29188