Detection of SARS‐CoV‐2 virus in middle ear effusions and its association with otitis media with effusion

A large‐scale outbreak of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) occurred in Shanghai, China, in early December 2022. To study the incidence and characteristics of otitis media with effusion (OME) complicating SARS‐CoV‐2, we collected 267 middle ear effusion (MEE) samples and 1...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of medical virology Jg. 96; H. 3; S. e29545 - n/a
Hauptverfasser: Fu, Xiao, Wang, Zhujian, Chen, Binjun, Sun, Haojie, Lyu, Jihan, Shao, Jing, Lu, Xiaoling, Xu, Jianghong, Yang, Juanmei, Chi, Fanglu, Huang, Yibo, Ren, Dongdong
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Wiley Subscription Services, Inc 01.03.2024
Schlagworte:
ACE2
Adult
Angiotensin
Angiotensin-Converting Enzyme 2
biostatistics & bioinformatics, data processing
biostatistics & bioinformatics, RNA extraction
China - epidemiology
coronavirus
Coronaviruses
COVID-19
COVID-19 - complications
Ear diseases
Effusion
Humans
Immunofluorescence
Load distribution
Middle ear
Otitis media
Otitis Media with Effusion
research and analysis methods
Respiratory diseases
Respiratory tract infection
SARS-CoV-2
Serine proteinase
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Tubes
Viral diseases
virus classification, data acquisition
Viruses
China
biostatistics & bioinformatics, RNA extraction
coronavirus
biostatistics & bioinformatics, data processing
virus classification, data acquisition
research and analysis methods
ISSN:0146-6615, 1096-9071, 1096-9071
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A large‐scale outbreak of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) occurred in Shanghai, China, in early December 2022. To study the incidence and characteristics of otitis media with effusion (OME) complicating SARS‐CoV‐2, we collected 267 middle ear effusion (MEE) samples and 172 nasopharyngeal (NP) swabs from patients. The SARS‐CoV‐2 virus was detected by RT‐PCR targeting. The SARS‐CoV‐2 virus, angiotensin‐converting enzyme 2 (ACE2), and transmembrane serine protease 2 (TMPRSS2) expression in human samples was examined via immunofluorescence. During the COVID‐19 epidemic in 2022, the incidence of OME (3%) significantly increased compared to the same period from 2020 to 2022. Ear symptoms in patients with SARS‐CoV‐2 complicated by OME generally appeared late, even after a negative NP swab, an average of 9.33 ± 6.272 days after COVID‐19 infection. The SARS‐CoV‐2 virus was detected in MEE, which had a higher viral load than NP swabs. The insertion rate of tympanostomy tubes was not significantly higher than in OME patients in 2019–2022. Virus migration led to high viral loads in MEE despite negative NP swabs, indicating that OME lagged behind respiratory infections but had a favorable prognosis. Furthermore, middle ear tissue from adult humans coexpressed the ACE2 receptor for the SARS‐CoV‐2 virus and the TMPRSS2 cofactors required for virus entry.
Bibliographie:Xiao Fu, Zhujian Wang, and Binjun Chen are contributed equally to this study.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:0146-6615
1096-9071
1096-9071
DOI:10.1002/jmv.29545