Mass spectrometry and the cellular surfaceome

The collection of exposed plasma membrane proteins, collectively termed the surfaceome, is involved in multiple vital cellular processes, such as the communication of cells with their surroundings and the regulation of transport across the lipid bilayer. The surfaceome also plays key roles in the im...

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Vydáno v:Mass spectrometry reviews Ročník 41; číslo 5; s. 804 - 841
Hlavní autoři: Pauwels, Jarne, Fijałkowska, Daria, Eyckerman, Sven, Gevaert, Kris
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Wiley Subscription Services, Inc 01.09.2022
Témata:
Antigens
enrichment strategies
Hydrophobicity
Immune system
LC‐MS/MS
Lipid bilayers
Mass spectrometry
Mass spectroscopy
Membrane proteins
plasma membrane proteins
Proteins
Proteomics
Scientific imaging
surfaceome
Therapeutic targets
enrichment strategies
plasma membrane proteins
LC-MS/MS
proteomics
surfaceome
ISSN:0277-7037, 1098-2787, 1098-2787
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Shrnutí:The collection of exposed plasma membrane proteins, collectively termed the surfaceome, is involved in multiple vital cellular processes, such as the communication of cells with their surroundings and the regulation of transport across the lipid bilayer. The surfaceome also plays key roles in the immune system by recognizing and presenting antigens, with its possible malfunctioning linked to disease. Surface proteins have long been explored as potential cell markers, disease biomarkers, and therapeutic drug targets. Despite its importance, a detailed study of the surfaceome continues to pose major challenges for mass spectrometry‐driven proteomics due to the inherent biophysical characteristics of surface proteins. Their inefficient extraction from hydrophobic membranes to an aqueous medium and their lower abundance compared to intracellular proteins hamper the analysis of surface proteins, which are therefore usually underrepresented in proteomic datasets. To tackle such problems, several innovative analytical methodologies have been developed. This review aims at providing an extensive overview of the different methods for surfaceome analysis, with respective considerations for downstream mass spectrometry‐based proteomics.
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ISSN:0277-7037
1098-2787
1098-2787
DOI:10.1002/mas.21690