The subbasement membrane distribution of type IV collagen in normal human skin

Samples of normal human skin were obtained from 48 sites in 26 subjects ranging in age from 2 to 85 years. The samples were examined by indirect immunofluorescence and immunoelectron microscopy using anti-human type IV collagen antibodies produced by immunizing rabbits with type IV collagen extracte...

Full description

Saved in:
Bibliographic Details
Published in:Journal of dermatology Vol. 16; no. 6; p. 458
Main Authors: Kobayashi, H, Ishii, M, Chanoki, M, Fukai, K, Hamada, T, Muragaki, Y, Ooshima, A
Format: Journal Article
Language:English
Published: England 01.12.1989
Subjects:
Adolescent
Adult
Aged
Basement Membrane - analysis
Child
Child, Preschool
Collagen - analysis
Female
Fluorescent Antibody Technique
Humans
Male
Microscopy, Electron
Middle Aged
Skin - analysis
Skin - ultrastructure
ISSN:0385-2407
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Samples of normal human skin were obtained from 48 sites in 26 subjects ranging in age from 2 to 85 years. The samples were examined by indirect immunofluorescence and immunoelectron microscopy using anti-human type IV collagen antibodies produced by immunizing rabbits with type IV collagen extracted from human placenta. Fluorescence was observed as granular or fine fibrous patterns, not only in the basement membrane at the dermo-epidermal junction, around the vessels, and the accessory organs of the skin, but also in the dermal regions in the vicinity of the basement membranes. This suggests the presence of type IV collagen in the dermis deep to the basement membrane. Ultrastructurally, the extrabasal lamina distribution of type IV collagen was noted as a partial distribution around the fibroblasts that existed close to the basal lamina. These findings are considered to be important in examining the function of this collagen in the dermis and the dynamics and metabolism of the basement membrane under normal and abnormal conditions.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0385-2407
DOI:10.1111/j.1346-8138.1989.tb01585.x