Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment

The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs. transformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopy has th...

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Veröffentlicht in:Analyst (London) Jg. 135; H. 12; S. 3266
Hauptverfasser: Trevisan, Júlio, Angelov, Plamen P, Patel, Imran I, Najand, Ghazal M, Cheung, Karen T, Llabjani, Valon, Pollock, Hubert M, Bruce, Shannon W, Pant, Kamala, Carmichael, Paul L, Scott, Andrew D, Martin, Francis L
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England 01.12.2010
Schlagworte:
Animals
Biological Assay - instrumentation
Biological Assay - methods
Cell Transformation, Neoplastic - drug effects
Cricetinae
Discriminant Analysis
Embryo, Mammalian - cytology
Embryo, Mammalian - drug effects
Mesocricetus - embryology
Organic Chemicals - pharmacology
Principal Component Analysis
Spectroscopy, Fourier Transform Infrared - instrumentation
Spectroscopy, Fourier Transform Infrared - methods
ISSN:1364-5528, 1364-5528
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Zusammenfassung:The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs. transformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopy has the potential to provide objective assessment of such SHE colonies with the added advantage of potentially providing mechanistic information. In this study, SHE cells were treated with one of eight different chemical regimens, allowed in culture to attach and form foci on IR-reflective glass slides; these were subsequently interrogated by attenuated total reflection (ATR) Fourier-transform IR (FTIR) spectroscopy. Derived mid-IR spectra (n = 13,406) were subjected to chemometric analysis focusing primarily on the extraction of biochemical information related to test agent treatment and/or morphological transformation. The use of ATR-FTIR spectroscopy with chemometrics to analyze the SHE assay is a novel approach to toxicological assessment.
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ISSN:1364-5528
1364-5528
DOI:10.1039/c0an00586j