Development of a Rapid On-Site Method for the Detection of Chicken Meat in Processed Ground Meat Products by Using a Direct Ultrafast PCR System

In this study, we developed a rapid on-site detection method by using direct ultrafast PCR coupled with a microfluidic chip to identify the presence of chicken meat in processed ground meat products. Chicken-specific PCR primer targeting mitochondrial 16S rRNA gene was newly designed, and its specif...

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Veröffentlicht in:	Journal of food protection Jg. 83; H. 6; S. 984 - 990
Hauptverfasser:	Sul, Suyeon, Kim, Mi-Ju, Lee, Jung-Min, Kim, Sung-Yeon, Kim, Hae-Yeong
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Elsevier Limited 01.06.2020
Schlagworte:	Animal species Animals autoclaving Beef Cattle chicken meat Chickens Commercialization Deoxyribonucleic acid Design detection limit Detection limits DNA Food Food safety genes ground meat Laboratories Meat Meat - analysis Meat products Meat Products - analysis Methods Microfluidics Mitochondria Onsite organ-on-a-chip Polymerase Chain Reaction Pork Poultry raw meat RNA, Ribosomal, 16S rRNA 16S Sample preparation South Korea Direct ultrafast PCR Meat adulteration On-site detection Processed ground meat product Chicken meat
ISSN:	0362-028X, 1944-9097, 1944-9097
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Abstract	In this study, we developed a rapid on-site detection method by using direct ultrafast PCR coupled with a microfluidic chip to identify the presence of chicken meat in processed ground meat products. Chicken-specific PCR primer targeting mitochondrial 16S rRNA gene was newly designed, and its specificity was confirmed against 17 other animal species and 4 different chicken meat samples from different countries of origin. The sensitivity of the chicken-specific ultrafast PCR was 0.1 pg of chicken DNA. To evaluate the limit of detection of the direct ultrafast PCR method, different percentages of chicken meat mixed with pork or beef were prepared. The limit of detection of the direct ultrafast PCR method for the chicken meat-pork and chicken meat-beef mixtures was 0.1% for both raw meat and autoclaved meat. This method was used for 15 commercialized processed ground meat products. In this method, the target sequence was successfully amplified, and the presence of chicken meat in processed ground meat products was identified within approximately 25 min, including the time for sample preparation. Thus, our study shows that this developed direct ultrafast PCR method is a rapid and accurate method for on-site detection of chicken DNA in commercial food products.
AbstractList	In this study, we developed a rapid on-site detection method by using direct ultrafast PCR coupled with a microfluidic chip to identify the presence of chicken meat in processed ground meat products. Chicken-specific PCR primer targeting mitochondrial 16S rRNA gene was newly designed, and its specificity was confirmed against 17 other animal species and 4 different chicken meat samples from different countries of origin. The sensitivity of the chicken-specific ultrafast PCR was 0.1 pg of chicken DNA. To evaluate the limit of detection of the direct ultrafast PCR method, different percentages of chicken meat mixed with pork or beef were prepared. The limit of detection of the direct ultrafast PCR method for the chicken meat–pork and chicken meat–beef mixtures was 0.1% for both raw meat and autoclaved meat. This method was used for 15 commercialized processed ground meat products. In this method, the target sequence was successfully amplified, and the presence of chicken meat in processed ground meat products was identified within approximately 25 min, including the time for sample preparation. Thus, our study shows that this developed direct ultrafast PCR method is a rapid and accurate method for on-site detection of chicken DNA in commercial food products.HIGHLIGHTSDirect ultrafast PCR system was developed for on-site chicken detection.Chicken-specific primers targeting the mitochondrial 16S rRNA gene were designed.Detection limits were 0.1% for both raw and autoclaved meats.Chicken meat in processed ground meat can be identified within approximately 25 min. In this study, we developed a rapid on-site detection method by using direct ultrafast PCR coupled with a microfluidic chip to identify the presence of chicken meat in processed ground meat products. Chicken-specific PCR primer targeting mitochondrial 16S rRNA gene was newly designed, and its specificity was confirmed against 17 other animal species and 4 different chicken meat samples from different countries of origin. The sensitivity of the chicken-specific ultrafast PCR was 0.1 pg of chicken DNA. To evaluate the limit of detection of the direct ultrafast PCR method, different percentages of chicken meat mixed with pork or beef were prepared. The limit of detection of the direct ultrafast PCR method for the chicken meat-pork and chicken meat-beef mixtures was 0.1% for both raw meat and autoclaved meat. This method was used for 15 commercialized processed ground meat products. In this method, the target sequence was successfully amplified, and the presence of chicken meat in processed ground meat products was identified within approximately 25 min, including the time for sample preparation. Thus, our study shows that this developed direct ultrafast PCR method is a rapid and accurate method for on-site detection of chicken DNA in commercial food products. In this study, we developed a rapid on-site detection method by using direct ultrafast PCR coupled with a microfluidic chip to identify the presence of chicken meat in processed ground meat products. Chicken-specific PCR primer targeting mitochondrial 16S rRNA gene was newly designed, and its specificity was confirmed against 17 other animal species and 4 different chicken meat samples from different countries of origin. The sensitivity of the chicken-specific ultrafast PCR was 0.1 pg of chicken DNA. To evaluate the limit of detection of the direct ultrafast PCR method, different percentages of chicken meat mixed with pork or beef were prepared. The limit of detection of the direct ultrafast PCR method for the chicken meat-pork and chicken meat-beef mixtures was 0.1% for both raw meat and autoclaved meat. This method was used for 15 commercialized processed ground meat products. In this method, the target sequence was successfully amplified, and the presence of chicken meat in processed ground meat products was identified within approximately 25 min, including the time for sample preparation. Thus, our study shows that this developed direct ultrafast PCR method is a rapid and accurate method for on-site detection of chicken DNA in commercial food products.ABSTRACTIn this study, we developed a rapid on-site detection method by using direct ultrafast PCR coupled with a microfluidic chip to identify the presence of chicken meat in processed ground meat products. Chicken-specific PCR primer targeting mitochondrial 16S rRNA gene was newly designed, and its specificity was confirmed against 17 other animal species and 4 different chicken meat samples from different countries of origin. The sensitivity of the chicken-specific ultrafast PCR was 0.1 pg of chicken DNA. To evaluate the limit of detection of the direct ultrafast PCR method, different percentages of chicken meat mixed with pork or beef were prepared. The limit of detection of the direct ultrafast PCR method for the chicken meat-pork and chicken meat-beef mixtures was 0.1% for both raw meat and autoclaved meat. This method was used for 15 commercialized processed ground meat products. In this method, the target sequence was successfully amplified, and the presence of chicken meat in processed ground meat products was identified within approximately 25 min, including the time for sample preparation. Thus, our study shows that this developed direct ultrafast PCR method is a rapid and accurate method for on-site detection of chicken DNA in commercial food products.
Author	Kim, Mi-Ju Lee, Jung-Min Kim, Sung-Yeon Kim, Hae-Yeong Sul, Suyeon
Author_xml	– sequence: 1 givenname: Suyeon surname: Sul fullname: Sul, Suyeon – sequence: 2 givenname: Mi-Ju surname: Kim fullname: Kim, Mi-Ju – sequence: 3 givenname: Jung-Min surname: Lee fullname: Lee, Jung-Min – sequence: 4 givenname: Sung-Yeon surname: Kim fullname: Kim, Sung-Yeon – sequence: 5 givenname: Hae-Yeong surname: Kim fullname: Kim, Hae-Yeong
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Cites_doi	10.1016/j.foodcont.2011.08.007 10.1016/j.foodcont.2013.04.014 10.1016/j.foodchem.2017.04.090 10.1002/jmv.25046 10.5740/jaoacint.14-230 10.1016/j.foodcont.2019.03.039 10.1080/10408398.2012.693978 10.1016/j.foodchem.2016.04.084 10.1016/j.trac.2016.02.026 10.1080/19440040903503070 10.1016/j.foodcont.2018.11.025 10.1016/j.meatsci.2009.06.003 10.1016/j.foodcont.2017.02.027 10.1007/s12024-013-9521-8 10.1016/j.meatsci.2014.06.006 10.1016/j.foodcont.2009.09.001 10.1016/j.foodcont.2017.09.012 10.1007/s10068-018-0479-x 10.5851/kosfa.2014.34.6.799 10.1371/journal.pone.0053325 10.1007/s00216-016-9668-8 10.1016/j.meatsci.2009.06.026 10.1016/j.foodchem.2018.07.138 10.1016/j.foodchem.2018.02.056 10.1016/j.foodcont.2016.01.041 10.1016/j.fsigss.2013.10.185
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References	Camma (10.4315/JFP-19-583_bb0025) 2012; 23 Kumar (10.4315/JFP-19-583_bb0100) 2015; 55 Haunshi (10.4315/JFP-19-583_bb0045) 2009; 83 Kim (10.4315/JFP-19-583_bb0060) 2016; 210 Wang (10.4315/JFP-19-583_bb0150) 2015; 98 Kim (10.4315/JFP-19-583_bb0065) 2018; 28 Ballin (10.4315/JFP-19-583_bb0015) 2009; 83 Kim (10.4315/JFP-19-583_bb0075) 2018; 85 Kitpipit (10.4315/JFP-19-583_bb0090) 2014; 10 Kim (10.4315/JFP-19-583_bb0085) 2018; 255 Thanakiatkrai (10.4315/JFP-19-583_bb0145) 2017; 233 Ahmed (10.4315/JFP-19-583_bb0010) 2010; 21 Kitpipit (10.4315/JFP-19-583_bb0095) 2013; 4 Danezis (10.4315/JFP-19-583_bb0035) 2016; 85 Kang (10.4315/JFP-19-583_bb0055) 2019; 271 Song (10.4315/JFP-19-583_bb0135) 2012; 7 Kim (10.4315/JFP-19-583_bb0080) 2019; 103 Natonek-Wisniewska (10.4315/JFP-19-583_bb0120) 2013; 34 Rojas (10.4315/JFP-19-583_bb0125) 2010; 27 Sul (10.4315/JFP-19-583_bb0140) 2019; 98 Jha (10.4315/JFP-19-583_bb0050) 2016 Kim (10.4315/JFP-19-583_bb0070) 2017; 78 Kwon (10.4315/JFP-19-583_bb0105) 2018; 90 Safdar (10.4315/JFP-19-583_bb0130) 2014; 98 Cho (10.4315/JFP-19-583_bb0030) 2014; 34 Lim (10.4315/JFP-19-583_bb0115) 2017; 32 Lee (10.4315/JFP-19-583_bb0110) 2016; 66 Bustin (10.4315/JFP-19-583_bb0020) 2017; 12 Furutani (10.4315/JFP-19-583_bb0040) 2016; 408
References_xml	– volume: 23 start-page: 400 year: 2012 ident: 10.4315/JFP-19-583_bb0025 article-title: Development and validation of fast real-time PCR assays for species identification in raw and cooked meat mixtures publication-title: Food Control doi: 10.1016/j.foodcont.2011.08.007 – volume: 34 start-page: 69 year: 2013 ident: 10.4315/JFP-19-583_bb0120 article-title: The species identification of bovine, porcine, ovine and chicken components in animal meals, feeds and their ingredients, based on COX I analysis and ribosomal DNA sequences publication-title: Food Control doi: 10.1016/j.foodcont.2013.04.014 – volume: 12 start-page: 10 year: 2017 ident: 10.4315/JFP-19-583_bb0020 article-title: How to speed up the polymerase chain reaction. Biomol. Detect publication-title: Quantif – volume: 233 start-page: 144 year: 2017 ident: 10.4315/JFP-19-583_bb0145 article-title: Meat species identification by two direct-triplex real-time PCR assays using low resolution melting publication-title: Food Chem doi: 10.1016/j.foodchem.2017.04.090 – volume: 90 start-page: 1019 year: 2018 ident: 10.4315/JFP-19-583_bb0105 article-title: A point-of-care diagnostic system to influenza viruses using chip-based ultra-fast PCR publication-title: J. Med. Virol doi: 10.1002/jmv.25046 – volume: 98 start-page: 410 year: 2015 ident: 10.4315/JFP-19-583_bb0150 article-title: Rapid visual detection of eight meat species using optical thin-film biosensor chips publication-title: J. AOAC Int doi: 10.5740/jaoacint.14-230 – volume: 103 start-page: 21 year: 2019 ident: 10.4315/JFP-19-583_bb0080 article-title: Development and validation of ultrafast PCR assays to detect six species of edible insects publication-title: Food Control doi: 10.1016/j.foodcont.2019.03.039 – volume: 55 start-page: 1340 year: 2015 ident: 10.4315/JFP-19-583_bb0100 article-title: Identification of species origin of meat and meat products on the DNA basis: a review publication-title: Crit. Rev. Food Sci. Nutr doi: 10.1080/10408398.2012.693978 – volume: 210 start-page: 102 year: 2016 ident: 10.4315/JFP-19-583_bb0060 article-title: Quantitative detection of pork in commercial meat products by TaqMan real-time PCR assay targeting the mitochondrial D-loop region publication-title: Food Chem doi: 10.1016/j.foodchem.2016.04.084 – volume: 85 start-page: 123 year: 2016 ident: 10.4315/JFP-19-583_bb0035 article-title: Food authentication: techniques, trends & emerging approaches publication-title: Trends Anal. Chem doi: 10.1016/j.trac.2016.02.026 – volume: 27 start-page: 749 year: 2010 ident: 10.4315/JFP-19-583_bb0125 article-title: Novel TaqMan real-time polymerase chain reaction assay for verifying the authenticity of meat and commercial meat products from game birds publication-title: Food Addit. Contam doi: 10.1080/19440040903503070 – volume: 98 start-page: 194 year: 2019 ident: 10.4315/JFP-19-583_bb0140 article-title: Development of a direct loop-mediated isothermal amplification (LAMP) assay for rapid and simple on-site detection of chicken in processed meat products publication-title: Food Control doi: 10.1016/j.foodcont.2018.11.025 – volume: 83 start-page: 165 year: 2009 ident: 10.4315/JFP-19-583_bb0015 article-title: Species determination – can we detect and quantify meat adulteration? publication-title: Meat Sci doi: 10.1016/j.meatsci.2009.06.003 – volume: 78 start-page: 1 year: 2017 ident: 10.4315/JFP-19-583_bb0070 article-title: Species identification of commercial jerky products in food and feed using direct pentaplex PCR assay publication-title: Food Control doi: 10.1016/j.foodcont.2017.02.027 – volume: 10 start-page: 29 year: 2014 ident: 10.4315/JFP-19-583_bb0090 article-title: Forensic animal DNA analysis using economical two-step direct PCR publication-title: Forensic Sci. Med. Pathol doi: 10.1007/s12024-013-9521-8 – volume: 32 start-page: 27 year: 2017 ident: 10.4315/JFP-19-583_bb0115 article-title: Development of ultra-rapid multiplex PCR detection against 6 major pathogens in honeybee publication-title: J. Apic – volume: 98 start-page: 296 year: 2014 ident: 10.4315/JFP-19-583_bb0130 article-title: A highly sensitive and specific tetraplex PCR assay for soybean, poultry, horse and pork species identification in sausages: development and validation publication-title: Meat Sci doi: 10.1016/j.meatsci.2014.06.006 – volume: 21 start-page: 599 year: 2010 ident: 10.4315/JFP-19-583_bb0010 article-title: Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensor publication-title: Food Control doi: 10.1016/j.foodcont.2009.09.001 – volume: 85 start-page: 1 year: 2018 ident: 10.4315/JFP-19-583_bb0075 article-title: Development of a fast duplex real-time PCR assay for simultaneous detection of chicken and pigeon in raw and heat-treated meats publication-title: Food Control doi: 10.1016/j.foodcont.2017.09.012 – volume: 28 start-page: 591 year: 2018 ident: 10.4315/JFP-19-583_bb0065 article-title: Rapid on-site detection of shrimp allergen tropomyosin using a novel ultrafast PCR system publication-title: Food Sci. Biotechnol doi: 10.1007/s10068-018-0479-x – volume: 34 start-page: 799 year: 2014 ident: 10.4315/JFP-19-583_bb0030 article-title: Meat species identification using loop-mediated isothermal amplification assay targeting species-specific mitochondrial DNA publication-title: Korean J. Food Sci. Anim. Resour doi: 10.5851/kosfa.2014.34.6.799 – volume: 7 start-page: e53325 year: 2012 ident: 10.4315/JFP-19-583_bb0135 article-title: Polymeric LabChip real-time PCR as a point-of-care-potential diagnostic tool for rapid detection of influenza A/H1N1 virus in human clinical specimens publication-title: PLoS One doi: 10.1371/journal.pone.0053325 – volume: 408 start-page: 5641 year: 2016 ident: 10.4315/JFP-19-583_bb0040 article-title: Development of an on-site rapid real-time polymerase chain reaction system and the characterization of suitable DNA polymerases for TaqMan probe technology publication-title: Anal. Bioanal. Chem doi: 10.1007/s00216-016-9668-8 – volume: 83 start-page: 454 year: 2009 ident: 10.4315/JFP-19-583_bb0045 article-title: Identification of chicken, duck, pigeon and pig meat by species-specific markers of mitochondrial origin publication-title: Meat Sci doi: 10.1016/j.meatsci.2009.06.026 – volume: 271 start-page: 1 year: 2019 ident: 10.4315/JFP-19-583_bb0055 article-title: Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus) publication-title: Food Chem doi: 10.1016/j.foodchem.2018.07.138 – volume: 255 start-page: 112 year: 2018 ident: 10.4315/JFP-19-583_bb0085 article-title: A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species publication-title: Food Chem doi: 10.1016/j.foodchem.2018.02.056 – start-page: 163 year: 2016 ident: 10.4315/JFP-19-583_bb0050 article-title: Spectroscopy and chemometrics – volume: 66 start-page: 53 year: 2016 ident: 10.4315/JFP-19-583_bb0110 article-title: Development of a rapid on-site detection method for pork in processed meat products using real-time loop-medicated isothermal amplification publication-title: Food Control doi: 10.1016/j.foodcont.2016.01.041 – volume: 4 start-page: e364 year: 2013 ident: 10.4315/JFP-19-583_bb0095 article-title: Direct PCR-FINS: wildlife species identification without DNA extraction publication-title: Forensic Sci. Int. Genet doi: 10.1016/j.fsigss.2013.10.185
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Snippet	In this study, we developed a rapid on-site detection method by using direct ultrafast PCR coupled with a microfluidic chip to identify the presence of chicken...
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SubjectTerms	Animal species Animals autoclaving Beef Cattle chicken meat Chickens Commercialization Deoxyribonucleic acid Design detection limit Detection limits DNA Food Food safety genes ground meat Laboratories Meat Meat - analysis Meat products Meat Products - analysis Methods Microfluidics Mitochondria Onsite organ-on-a-chip Polymerase Chain Reaction Pork Poultry raw meat RNA, Ribosomal, 16S rRNA 16S Sample preparation
Title	Development of a Rapid On-Site Method for the Detection of Chicken Meat in Processed Ground Meat Products by Using a Direct Ultrafast PCR System
URI	https://www.ncbi.nlm.nih.gov/pubmed/32034408 https://www.proquest.com/docview/2466046347 https://www.proquest.com/docview/2352656948 https://www.proquest.com/docview/2524282581
Volume	83
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