Development of a Rapid On-Site Method for the Detection of Chicken Meat in Processed Ground Meat Products by Using a Direct Ultrafast PCR System

In this study, we developed a rapid on-site detection method by using direct ultrafast PCR coupled with a microfluidic chip to identify the presence of chicken meat in processed ground meat products. Chicken-specific PCR primer targeting mitochondrial 16S rRNA gene was newly designed, and its specif...

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Vydáno v:Journal of food protection Ročník 83; číslo 6; s. 984 - 990
Hlavní autoři: Sul, Suyeon, Kim, Mi-Ju, Lee, Jung-Min, Kim, Sung-Yeon, Kim, Hae-Yeong
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Elsevier Limited 01.06.2020
Témata:
Animal species
Animals
autoclaving
Beef
Cattle
chicken meat
Chickens
Commercialization
Deoxyribonucleic acid
Design
detection limit
Detection limits
DNA
Food
Food safety
genes
ground meat
Laboratories
Meat
Meat - analysis
Meat products
Meat Products - analysis
Methods
Microfluidics
Mitochondria
Onsite
organ-on-a-chip
Polymerase Chain Reaction
Pork
Poultry
raw meat
RNA, Ribosomal, 16S
rRNA 16S
Sample preparation
South Korea
Direct ultrafast PCR
Meat adulteration
On-site detection
Processed ground meat product
Chicken meat
ISSN:0362-028X, 1944-9097, 1944-9097
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Shrnutí:In this study, we developed a rapid on-site detection method by using direct ultrafast PCR coupled with a microfluidic chip to identify the presence of chicken meat in processed ground meat products. Chicken-specific PCR primer targeting mitochondrial 16S rRNA gene was newly designed, and its specificity was confirmed against 17 other animal species and 4 different chicken meat samples from different countries of origin. The sensitivity of the chicken-specific ultrafast PCR was 0.1 pg of chicken DNA. To evaluate the limit of detection of the direct ultrafast PCR method, different percentages of chicken meat mixed with pork or beef were prepared. The limit of detection of the direct ultrafast PCR method for the chicken meat-pork and chicken meat-beef mixtures was 0.1% for both raw meat and autoclaved meat. This method was used for 15 commercialized processed ground meat products. In this method, the target sequence was successfully amplified, and the presence of chicken meat in processed ground meat products was identified within approximately 25 min, including the time for sample preparation. Thus, our study shows that this developed direct ultrafast PCR method is a rapid and accurate method for on-site detection of chicken DNA in commercial food products.
Bibliografie:ObjectType-Article-1
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ISSN:0362-028X
1944-9097
1944-9097
DOI:10.4315/JFP-19-583