Phagocyte-mediated killing of Candida tropicalis

Human peripheral monocytes (MO), neutrophils (PMN), and lymphocytes (PBL) were tested for their ability to kill Candida tropicalis. With incubation times between 30 min and 2 h, unstimulated MO and PMN, but not PBL, were efficient killers of C. tropicalis. Both leukocyte subsets were able to kill at...

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Bibliographic Details
Published in:Mycopathologia (1975) Vol. 113; no. 2; p. 81
Main Authors: Lindemann, R A, Franker, C K
Format: Journal Article
Language:English
Published: Netherlands 01.02.1991
Subjects:
Candida - immunology
Catalase - pharmacology
Cells, Cultured
Humans
Interferon-gamma - pharmacology
Interleukin-2 - pharmacology
Kinetics
Lymphocytes - immunology
Monocytes - immunology
Neutrophils - immunology
Phagocytosis
Superoxide Dismutase - pharmacology
Tumor Necrosis Factor-alpha - pharmacology
ISSN:0301-486X
Online Access:Get more information
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Summary:Human peripheral monocytes (MO), neutrophils (PMN), and lymphocytes (PBL) were tested for their ability to kill Candida tropicalis. With incubation times between 30 min and 2 h, unstimulated MO and PMN, but not PBL, were efficient killers of C. tropicalis. Both leukocyte subsets were able to kill at minimum 2.5:1 effector to target ratios. Pre-incubation of MO for 24 h with interferon-gamma or tumor necrosis factor (TNF) increased their ability to kill yeast targets. TNF alone had no effect on C. tropicalis targets at concentrations up to 1000 U/ml. PBL activated for 4 d with interleukin-2 did not kill yeast targets. PMN exhibited more cytocidal efficiency per cell than MO in these assays. Direct contact of effectors and targets was required; no significant killing by PMN or MO supernatants was measured. PMN-mediated killing, but not MO killing, was inhibited by a mixture of catalase and superoxide dismutase suggesting that oxygen-dependent killing mechanisms were partially responsible for candidacidal activity.
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ISSN:0301-486X
DOI:10.1007/BF00442414