Platelet-derived growth factor BB is reduced in endometrial endothelial cells of women with abnormal uterine bleeding–endometrial disorder

What is the expression pattern of platelet-derived growth factor BB (PDGF-BB), and its receptors, across the menstrual cycle in healthy control women and those with abnormal uterine bleeding–endometrial disorder (AUB-E)? Immunohistochemical staining for PDGF-BB, platelet-derived growth factor recept...

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Vydané v:Reproductive biomedicine online Ročník 45; číslo 3; s. 531 - 543
Hlavní autori: Biswas Shivhare, Sourima, Lu, Qinsheng, Sun, Dingqian, Hou, Huomei, Bulmer, Judith N., Innes, Barbara A., Hapangama, Dharani K., Lash, Gendie E.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Netherlands Elsevier Ltd 01.09.2022
Predmet:
Abnormal uterine bleeding
Becaplermin - metabolism
Cells, Cultured
Endometrium
Endometrium - metabolism
Endometrium - physiopathology
Endothelial Cells
Female
Humans
Intercellular Signaling Peptides and Proteins - metabolism
Obstetrics and Gynecology
Platelet-derived growth factor BB
Receptor, Platelet-Derived Growth Factor alpha - metabolism
Uterine Diseases
Uterine Hemorrhage
VSMC differentiation
Abnormal uterine bleeding
VSMC differentiation
Platelet-derived growth factor BB
Endometrium
Endothelial cells
endothelial cells
platelet derived growth factor BB
abnormal uterine bleeding
ISSN:1472-6483, 1472-6491, 1472-6491
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Shrnutí:What is the expression pattern of platelet-derived growth factor BB (PDGF-BB), and its receptors, across the menstrual cycle in healthy control women and those with abnormal uterine bleeding–endometrial disorder (AUB-E)? Immunohistochemical staining for PDGF-BB, platelet-derived growth factor receptor alpha (PDGFRα) and platelet-derived growth factor beta (PDGFRβ) was performed in control and AUB-E endometrium from the proliferative, early, mid- and late secretory phases of the menstrual cycle (n = 5 each group). Control proliferative phase endometrium was cultured in PDGF-BB (0, 10 ng/ml) and vascular maturation assessed (n = 3). Endothelial cell to vascular smooth muscle cell (VSMC) association was assessed after treatment with PDGF-BB (0, 1, 10 ng/ml). Secretion of angiogenic growth factors by endothelial cells or VSMC was determined. Endothelial cell immunoreactivity for PDGF-BB was reduced in the mid and late secretory phases in AUB-E (P = 0.008). PDGFRα was also reduced in mid secretory phase endothelial cells, proliferative and early secretory phase glandular epithelium in AUB-E (P = 0.008). PDGFRβ expression was not altered. Treatment of proliferative phase endometrium with PDGF-BB (10 ng/ml) reduced the percentage of vessels expressing contractile VSMC markers. PDGF-BB had no effect on angiogenic growth factor secretion by endothelial cells or VSMC in vitro and did not affect their association in an in-vitro endothelial cell–VSMC association assay. Reduced endothelial cell expression of PDGF-BB in the AUB-E endometrium may contribute to the reduced vascular maturation previously observed in these women.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1472-6483
1472-6491
1472-6491
DOI:10.1016/j.rbmo.2022.02.005