Designing of putative siRNA against geminiviral suppressors of RNAi to develop geminivirus-resistant papaya crop

Geminiviruses are single-stranded circular DNA viruses causing leaf curl disease in papaya crop. Post-Transcriptional Gene Silencing (PTGS), also known as RNAi, acts as a natural antiviral defence mechanism and plays a role in genome maintenance and development in plants. PTGS suppression by viruses...

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Bibliographic Details
Published in:International journal of bioinformatics research and applications Vol. 9; no. 1; p. 3
Main Authors: Saxena, Sangeeta, Kesharwani, Rupesh K, Singh, Vinayak, Singh, Sarita
Format: Journal Article
Language:English
Published: Switzerland 2013
Subjects:
Carica - genetics
Carica - virology
Geminiviridae - genetics
Geminiviridae - pathogenicity
Genes, Viral
Plant Diseases - genetics
Plant Diseases - virology
RNA Interference
RNA, Small Interfering - genetics
Viral Proteins - genetics
Viral Proteins - metabolism
ISSN:1744-5485
Online Access:Get more information
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Summary:Geminiviruses are single-stranded circular DNA viruses causing leaf curl disease in papaya crop. Post-Transcriptional Gene Silencing (PTGS), also known as RNAi, acts as a natural antiviral defence mechanism and plays a role in genome maintenance and development in plants. PTGS suppression by viruses makes the plant RNA silencing machinery inefficient. Three geminiviral genes namely AV2, AC2 and AC4 are found to play the role in suppression of RNA silencing. siRNA degrades the target mRNA in a homology-dependent manner. In-silico designing of siRNA against these three genes of geminiviruses infecting Carica papaya was done using bioinformatics tools. This strategy may provide PTGS by specifically targeting the viral genes involved in suppression of plant RNA silencing machinery.
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ISSN:1744-5485
DOI:10.1504/IJBRA.2013.050655