A NEW FACILE METHOD FOR SPIN-LABELING OF OLIGONUCLEOTIDES

A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the...

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Bibliographic Details

Published in:	Nucleosides, nucleotides & nucleic acids Vol. 20; no. 4-7; pp. 1271 - 1274
Main Authors:	Strube, Thorsten, Schiemann, Olav, MacMillan, Fraser, Prisner, Thomas, Engels, Joachim W.
Format:	Journal Article
Language:	English
Published:	United States Taylor & Francis Group 01.04.2001
Subjects:	DNA - chemical synthesis DNA - chemistry Nucleic Acid Conformation Oligonucleotides - chemical synthesis Oligonucleotides - chemistry RNA - chemical synthesis RNA - chemistry Spin Labels - chemical synthesis
ISSN:	1525-7770, 1532-2335
Online Access:	Get full text
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