Workshop cluster 1+γδ T‐cell receptor+ T cells from calves express high levels of interferon‐γ in response to stimulation with interleukin‐12 and ‐18

Summary γδ T‐cell receptor+ T lymphocytes are an important element of the innate immune system. Early production of interferon (IFN)‐γ by γδ T cells may have a role in linking innate and adaptive immune responses and contribute to T helper‐1 bias. We investigated the role of cytokines in the activat...

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Bibliographic Details
Published in:Immunology Vol. 120; no. 1; pp. 57 - 65
Main Authors: Price, Sally J., Sopp, Paul, Howard, Christopher J., Hope, Jayne C.
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01.01.2007
Blackwell Publishing Inc
Subjects:
bovine
interferon‐γ
interleukin‐12
neonatal
Original
workshop cluster 1+γδ T cells
ISSN:0019-2805, 1365-2567
Online Access:Get full text
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Summary:Summary γδ T‐cell receptor+ T lymphocytes are an important element of the innate immune system. Early production of interferon (IFN)‐γ by γδ T cells may have a role in linking innate and adaptive immune responses and contribute to T helper‐1 bias. We investigated the role of cytokines in the activation and induction of IFN‐γ secretion by bovine workshop cluster 1+ (WC1+) γδ T cells. The effects of culture with interleukin (IL)‐12, IL‐18, IL‐15 and IL‐2 were investigated; these cytokines are known to influence murine and human γδ T cells. We report that bovine WC1+γδ T cells are synergistically stimulated by IL‐12 and IL‐18 to secrete large quantities of IFN‐γ. Neonatal calves were shown to have significantly higher numbers of circulating WC1+γδ T cells than adult animals. In addition, the response of peripheral blood WC1+γδ T cells was significantly higher in neonatal calves compared with adult animals. However, in adult animals the response of lymph node WC1+γδ T cells to IL‐12/IL‐18 was more pronounced than that of peripheral blood WC1+γδ T cells. We hypothesize that the induction of IFN‐γ secretion from WC1+γδ T cells by IL‐12 and IL‐18 is likely to be an important element of the innate response to pathogens such as Mycobacterium bovis. The high numbers of WC1+γδ T cells in neonatal calves, and their inherent ability to respond to inflammatory cytokines, could be a key factor in the enhanced responses seen in calves to BCG vaccination.
ISSN:0019-2805
1365-2567
DOI:10.1111/j.1365-2567.2006.02477.x