Analysis of the expression of FAP‐α protein in 2D‐keloid fibroblast cultures and in 3D models of keloid

Objective Keloids arise most often as a result of abnormal wound healing. The lesion characterizes inflammation and fibroproliferation. Fibroblast activation protein alpha (FAP‐α) is one of enzymes engaged in keloid formation. It is serine protease that facilitates cells invasion and growth. FAP‐α p...

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Vydáno v:Journal of cosmetic dermatology Ročník 21; číslo 8; s. 3561 - 3566
Hlavní autoři: Katarzyna, Adamczyk, Agnieszka, Garncarczyk, Paweł, Antończak, Magdalena, Kimsa‐Dudek, Agnieszka, Synowiec‐Wojtarowicz, Małgorzata, Derewniuk, Agnieszka, Lubczyńska, Dominika, Wcisło‐Dziadecka
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 01.08.2022
Témata:
3D model
FAP‐α
fibroblast activating protein α
keloid
FAP-α
fibroblast activating protein α
keloid
3D model
ISSN:1473-2130, 1473-2165, 1473-2165
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Shrnutí:Objective Keloids arise most often as a result of abnormal wound healing. The lesion characterizes inflammation and fibroproliferation. Fibroblast activation protein alpha (FAP‐α) is one of enzymes engaged in keloid formation. It is serine protease that facilitates cells invasion and growth. FAP‐α possess also non‐enzymatic activity and plays role in activation of cell signaling. The aim of the study was to assess the impact of culture conditions on the proliferation of keloid fibroblasts and the expression of FAP‐α. Analysis of utility of 3D models of keloid in in vitro study of pathogenesis of keloids was also made. Methods NHDF and KEL FIB cells were cultured in vitro in 2D cultures and 3D Matrigel models. The viability of cells was assayed spectrophotometrically with WST‐1 test. FAP‐α protein amount in cell cultures and 3D models was evaluated with the use of ELISA test. Results KEL FIB fibroblasts exhibited higher viability than NHDF fibroblasts in all three models of keloid. The expression of FAP‐α is different in normal and keloid fibroblasts. In vitro conditions influence the expression of FAP‐α in NHDF cells but not in KEL FIB cells. Conclusions This preliminary study has shown that the expression of FAP‐α, similarly to other enzymes engaged in keloid formation, is different in keloids in vivo and in in vitro models. FAP‐α expression is modulated by in vitro conditions in normal fibroblasts but not in keloid fibroblasts.
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ISSN:1473-2130
1473-2165
1473-2165
DOI:10.1111/jocd.14667