Monoclonal antibodies protect against pandrug-resistant Klebsiella pneumoniae
The ‘silent pandemic’ caused by antimicrobial resistance requires innovative therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most transformative and safe drugs in oncology 1 and autoimmunity 2 , are rarely used for infectious diseases and not yet used for antimicrobia...
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| Published in: | Nature (London) Vol. 646; no. 8087; pp. 1204 - 1213 |
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
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London
Nature Publishing Group UK
30.10.2025
Nature Publishing Group |
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| ISSN: | 0028-0836, 1476-4687, 1476-4687 |
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| Abstract | The ‘silent pandemic’ caused by antimicrobial resistance requires innovative therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most transformative and safe drugs in oncology
1
and autoimmunity
2
, are rarely used for infectious diseases and not yet used for antimicrobial resistance
3
. Here we applied an antigen-agnostic strategy to isolate extremely potent human mAbs against
Klebsiella pneumoniae
sequence type 147 (ST147), a hypervirulent and pandrug-resistant lineage that is spreading globally. Isolated mAbs target the KL64 capsule and the O-antigen. However, although mAbs displayed bactericidal activity in the picomolar range in vitro, only the capsule-specific mAbs were protective against fulminant bloodstream infection by ST147 and two geographically and genetically distant carbapenem-resistant KL64-bearing
K. pneumoniae
. Protection observed in vivo correlated with in vitro bacterial uptake by macrophages and enchained bacterial growth. Our study thus describes a mAb that protects against pandrug-resistant
K. pneumoniae
and provides a strategy to isolate mAbs and identify mAbs that confer protection against bacteria with antimicrobial resistance.
Capsule-specific human monoclonal antibodies are protective against bloodstream infection by
Klebsiella pneumoniae
sequence type 147 in a mouse model of septicaemia, and could provide a useful strategy against pathogens with antimicrobial resistance. |
|---|---|
| AbstractList | The 'silent pandemic' caused by antimicrobial resistance requires innovative therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most transformative and safe drugs in oncology
and autoimmunity
, are rarely used for infectious diseases and not yet used for antimicrobial resistance
. Here we applied an antigen-agnostic strategy to isolate extremely potent human mAbs against Klebsiella pneumoniae sequence type 147 (ST147), a hypervirulent and pandrug-resistant lineage that is spreading globally. Isolated mAbs target the KL64 capsule and the O-antigen. However, although mAbs displayed bactericidal activity in the picomolar range in vitro, only the capsule-specific mAbs were protective against fulminant bloodstream infection by ST147 and two geographically and genetically distant carbapenem-resistant KL64-bearing K. pneumoniae. Protection observed in vivo correlated with in vitro bacterial uptake by macrophages and enchained bacterial growth. Our study thus describes a mAb that protects against pandrug-resistant K. pneumoniae and provides a strategy to isolate mAbs and identify mAbs that confer protection against bacteria with antimicrobial resistance. The ‘silent pandemic’ caused by antimicrobial resistance requires innovative therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most transformative and safe drugs in oncology 1 and autoimmunity 2 , are rarely used for infectious diseases and not yet used for antimicrobial resistance 3 . Here we applied an antigen-agnostic strategy to isolate extremely potent human mAbs against Klebsiella pneumoniae sequence type 147 (ST147), a hypervirulent and pandrug-resistant lineage that is spreading globally. Isolated mAbs target the KL64 capsule and the O-antigen. However, although mAbs displayed bactericidal activity in the picomolar range in vitro, only the capsule-specific mAbs were protective against fulminant bloodstream infection by ST147 and two geographically and genetically distant carbapenem-resistant KL64-bearing K. pneumoniae . Protection observed in vivo correlated with in vitro bacterial uptake by macrophages and enchained bacterial growth. Our study thus describes a mAb that protects against pandrug-resistant K. pneumoniae and provides a strategy to isolate mAbs and identify mAbs that confer protection against bacteria with antimicrobial resistance. Capsule-specific human monoclonal antibodies are protective against bloodstream infection by Klebsiella pneumoniae sequence type 147 in a mouse model of septicaemia, and could provide a useful strategy against pathogens with antimicrobial resistance. The 'silent pandemic' caused by antimicrobial resistance requires innovative therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most transformative and safe drugs in oncology1 and autoimmunity2, are rarely used for infectious diseases and not yet used for antimicrobial resistance3. Here we applied an antigen-agnostic strategy to isolate extremely potent human mAbs against Klebsiella pneumoniae sequence type 147 (ST147), a hypervirulent and pandrug-resistant lineage that is spreading globally. Isolated mAbs target the KL64 capsule and the O-antigen. However, although mAbs displayed bactericidal activity in the picomolar range in vitro, only the capsule-specific mAbs were protective against fulminant bloodstream infection by ST147 and two geographically and genetically distant carbapenem-resistant KL64-bearing K. pneumoniae. Protection observed in vivo correlated with in vitro bacterial uptake by macrophages and enchained bacterial growth. Our study thus describes a mAb that protects against pandrug-resistant K. pneumoniae and provides a strategy to isolate mAbs and identify mAbs that confer protection against bacteria with antimicrobial resistance.The 'silent pandemic' caused by antimicrobial resistance requires innovative therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most transformative and safe drugs in oncology1 and autoimmunity2, are rarely used for infectious diseases and not yet used for antimicrobial resistance3. Here we applied an antigen-agnostic strategy to isolate extremely potent human mAbs against Klebsiella pneumoniae sequence type 147 (ST147), a hypervirulent and pandrug-resistant lineage that is spreading globally. Isolated mAbs target the KL64 capsule and the O-antigen. However, although mAbs displayed bactericidal activity in the picomolar range in vitro, only the capsule-specific mAbs were protective against fulminant bloodstream infection by ST147 and two geographically and genetically distant carbapenem-resistant KL64-bearing K. pneumoniae. Protection observed in vivo correlated with in vitro bacterial uptake by macrophages and enchained bacterial growth. Our study thus describes a mAb that protects against pandrug-resistant K. pneumoniae and provides a strategy to isolate mAbs and identify mAbs that confer protection against bacteria with antimicrobial resistance. The ‘silent pandemic’ caused by antimicrobial resistance requires innovative therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most transformative and safe drugs in oncology1 and autoimmunity2, are rarely used for infectious diseases and not yet used for antimicrobial resistance3. Here we applied an antigen-agnostic strategy to isolate extremely potent human mAbs against Klebsiella pneumoniae sequence type 147 (ST147), a hypervirulent and pandrug-resistant lineage that is spreading globally. Isolated mAbs target the KL64 capsule and the O-antigen. However, although mAbs displayed bactericidal activity in the picomolar range in vitro, only the capsule-specific mAbs were protective against fulminant bloodstream infection by ST147 and two geographically and genetically distant carbapenem-resistant KL64-bearing K. pneumoniae. Protection observed in vivo correlated with in vitro bacterial uptake by macrophages and enchained bacterial growth. Our study thus describes a mAb that protects against pandrug-resistant K. pneumoniae and provides a strategy to isolate mAbs and identify mAbs that confer protection against bacteria with antimicrobial resistance.Capsule-specific human monoclonal antibodies are protective against bloodstream infection by Klebsiella pneumoniae sequence type 147 in a mouse model of septicaemia, and could provide a useful strategy against pathogens with antimicrobial resistance. |
| Author | Bosch, Soraya Soledad Tiseo, Giusy Zucconi Galli Fonseca, Vittoria Paciello, Ida Abdelraouf, Kamilia De Santi, Concetta Giordano, Cesira Stazzoni, Samuele Dortet, Laurent Kabanova, Anna Nicolau, David P. Yuwei, Shen Batani, Giampiero Barnini, Simona Menichetti, Francesco Ciabattini, Annalisa Ridelfi, Matteo Rappuoli, Rino Cerofolini, Linda Capoccia, Laura Cardinali, Giulia Mugnaini, Chiara Sala, Claudia Fragai, Marco Roscioli, Emanuele Maccari, Giuseppe Troisi, Marco Licastro, Danilo Wyres, Kelly Falcone, Marco Cardamone, Dario Manganaro, Noemi |
| Author_xml | – sequence: 1 givenname: Emanuele orcidid: 0000-0003-4125-4632 surname: Roscioli fullname: Roscioli, Emanuele organization: Monoclonal Antibody Discovery (MAD) Lab, Fondazione Toscana Life Sciences – sequence: 2 givenname: Vittoria orcidid: 0000-0002-4593-2544 surname: Zucconi Galli Fonseca fullname: Zucconi Galli Fonseca, Vittoria organization: Monoclonal Antibody Discovery (MAD) Lab, Fondazione Toscana Life Sciences, Department of Biotechnology, Chemistry and Pharmacy, University of Siena – sequence: 3 givenname: Soraya Soledad orcidid: 0000-0003-1459-4463 surname: Bosch fullname: Bosch, Soraya Soledad organization: Monoclonal Antibody Discovery (MAD) Lab, Fondazione Toscana Life Sciences – sequence: 4 givenname: Ida surname: Paciello fullname: Paciello, Ida organization: Monoclonal Antibody Discovery (MAD) Lab, Fondazione Toscana Life Sciences – sequence: 5 givenname: Giuseppe surname: Maccari fullname: Maccari, Giuseppe organization: Data Science for Health (DASCH) Lab, Fondazione Toscana Life Sciences – sequence: 6 givenname: Giulia surname: Cardinali fullname: Cardinali, Giulia organization: Monoclonal Antibody Discovery (MAD) Lab, Fondazione Toscana Life Sciences, Department of Biotechnology, Chemistry and Pharmacy, University of Siena – sequence: 7 givenname: Giampiero surname: Batani fullname: Batani, Giampiero organization: Monoclonal Antibody Discovery (MAD) Lab, Fondazione Toscana Life Sciences – sequence: 8 givenname: Samuele orcidid: 0000-0002-1579-6895 surname: Stazzoni fullname: Stazzoni, Samuele organization: Monoclonal Antibody Discovery (MAD) Lab, Fondazione Toscana Life Sciences – sequence: 9 givenname: Giusy surname: Tiseo fullname: Tiseo, Giusy organization: Department of Clinical and Experimental Medicine, Infectious Diseases Unit, University of Pisa – sequence: 10 givenname: Cesira surname: Giordano fullname: Giordano, Cesira organization: Microbiology Unit, Azienda Universitaria Ospedaliera Pisana – sequence: 11 givenname: 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– sequence: 31 givenname: Anna orcidid: 0000-0002-2077-472X surname: Kabanova fullname: Kabanova, Anna email: a.kabanova@toscanalifesciences.org organization: Monoclonal Antibody Discovery (MAD) Lab, Fondazione Toscana Life Sciences, Tumor Immunology Unit, Fondazione Toscana Life Sciences – sequence: 32 givenname: Rino orcidid: 0000-0002-8827-254X surname: Rappuoli fullname: Rappuoli, Rino email: rino.rappuoli@biotecnopolo.it organization: Fondazione Biotecnopolo di Siena |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/41034577$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/S0008-6215(00)84780-1 10.1016/S1473-3099(23)00572-8 10.3390/biology13040256 10.1016/j.cell.2016.03.017 10.1128/IAI.00693-20 10.1016/j.ijmm.2017.09.012 10.1128/IAI.00678-15 10.1016/j.ijantimicag.2004.10.001 10.1074/jbc.M110.122598 10.1074/jbc.RA117.000646 10.1073/pnas.1717162115 10.1038/s41579-020-00506-3 10.1097/01.CCM.0000217961.75225.E9 10.1371/journal.pcbi.1006986 10.1093/infdis/jiw021 10.3389/fcimb.2023.1108818 10.1038/s41590-018-0106-2 10.2807/1560-7917.ES.2020.25.48.2001779 10.3389/fmicb.2021.691406 10.1080/19420862.2021.2006123 10.3389/fmicb.2022.1080059 10.1074/jbc.M202683200 10.1371/journal.pone.0056847 10.1186/s13073-019-0706-y 10.3390/antibiotics10060691 10.1099/mic.0.28285-0 10.1016/0008-6215(87)80283-5 10.1128/jb.108.3.1304-1309.1971 10.1128/IAI.70.5.2583-2590.2002 10.1093/cid/ciaa586 10.1172/jci.insight.92774 10.1128/IAI.68.5.2402-2409.2000 10.1007/s00262-024-03814-2 10.1016/S2666-5247(21)00268-8 10.1016/j.cmi.2023.11.005 10.1371/journal.pbio.1002344 10.1038/s41467-017-02223-7 10.1016/j.intimp.2021.108305 10.3390/biotech10030019 10.1371/journal.ppat.1011367 10.3201/eid2602.190594 10.1016/j.cell.2021.02.035 10.1099/mgen.0.000102 10.1099/jmm.0.001823 10.1128/AAC.01074-18 10.1128/CVI.00456-16 10.3947/ic.2017.49.1.57 10.1093/cid/ciab457 10.1099/mgen.0.000967 |
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| References | Q Wang (9391_CR22) 2016; 213 K Zhou (9391_CR35) 2020; 26 P Starkova (9391_CR33) 2021; 10 V Kos (9391_CR26) 2010; 285 ME Pennini (9391_CR16) 2017; 8 G Cortés (9391_CR39) 2002; 70 K Abdelraouf (9391_CR30) 2018; 62 N Ravenscroft (9391_CR54) 1987; 167 SK Berry (9391_CR43) 2022; 14 VD Pilato (9391_CR8) 2022; 3 E Diago-Navarro (9391_CR19) 2018; 9 MG Aruta (9391_CR23) 2021; 10 SZ Mirjalili (9391_CR2) 2021; 101 W Wang (9391_CR25) 2023; 13 V Regueiro (9391_CR40) 2006; 152 TS Cohen (9391_CR17) 2017; 2 Z Lapp (9391_CR32) 2021; 73 EH Merrifield (9391_CR27) 1979; 74 J Wang (9391_CR46) 2017; 307 EJ Klemm (9391_CR5) 2018; 115 C Tascini (9391_CR10) 2024; 30 JF Turton (9391_CR13) 2024; 73 PL Wantuch (9391_CR21) 2023; 19 MJ Martin (9391_CR12) 2023; 9 C March (9391_CR41) 2013; 8 9391_CR3 BR Clarke (9391_CR28) 2018; 293 RN De Jong (9391_CR29) 2016; 14 K Toledo-Stuardo (9391_CR1) 2024; 73 BJ Gormus (9391_CR51) 1971; 108 9391_CR4 M Troisi (9391_CR14) 2022; 13 M Falcone (9391_CR24) 2020; 25 E Andreano (9391_CR50) 2021; 184 F Nonne (9391_CR55) 2024; 13 TK Held (9391_CR42) 2000; 68 S Gonzalez-Ferrer (9391_CR6) 2021; 89 MJ Martin (9391_CR9) 2021; 118 E Diago-Navarro (9391_CR20) 2017; 24 M Falcone (9391_CR7) 2021; 72 A Kumar (9391_CR38) 2006; 34 H Xiong (9391_CR45) 2016; 165 K Banerjee (9391_CR15) 2022; 10 T Rollenske (9391_CR18) 2018; 19 F Bansept (9391_CR48) 2019; 15 J Wassil (9391_CR44) 2024; 24 F Micoli (9391_CR52) 2021; 19 E Vinogradov (9391_CR53) 2002; 277 H Xiong (9391_CR47) 2015; 83 9391_CR49 JY Park (9391_CR31) 2017; 49 KL Wyres (9391_CR34) 2020; 12 D Zhao (9391_CR36) 2021; 12 J Li (9391_CR11) 2005; 25 J Wang (9391_CR37) 2023; 8 |
| References_xml | – volume: 74 start-page: 241 year: 1979 ident: 9391_CR27 publication-title: Carbohydr. Res. doi: 10.1016/S0008-6215(00)84780-1 – volume: 24 start-page: 308 year: 2024 ident: 9391_CR44 publication-title: Lancet Infect. Dis. doi: 10.1016/S1473-3099(23)00572-8 – volume: 13 start-page: 256 year: 2024 ident: 9391_CR55 publication-title: Biology doi: 10.3390/biology13040256 – volume: 165 start-page: 679 year: 2016 ident: 9391_CR45 publication-title: Cell doi: 10.1016/j.cell.2016.03.017 – volume: 89 year: 2021 ident: 9391_CR6 publication-title: Infect. Immun. doi: 10.1128/IAI.00693-20 – volume: 9 year: 2018 ident: 9391_CR19 publication-title: mBio – volume: 307 start-page: 490 year: 2017 ident: 9391_CR46 publication-title: Int. J. Med. Microbiol. doi: 10.1016/j.ijmm.2017.09.012 – volume: 83 start-page: 3418 year: 2015 ident: 9391_CR47 publication-title: Infect. Immun. doi: 10.1128/IAI.00678-15 – volume: 25 start-page: 11 year: 2005 ident: 9391_CR11 publication-title: Int. J. Antimicrob. Agents doi: 10.1016/j.ijantimicag.2004.10.001 – volume: 285 start-page: 19668 year: 2010 ident: 9391_CR26 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M110.122598 – volume: 293 start-page: 4666 year: 2018 ident: 9391_CR28 publication-title: J. Biol. Chem. doi: 10.1074/jbc.RA117.000646 – ident: 9391_CR4 – volume: 115 start-page: 12872 year: 2018 ident: 9391_CR5 publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.1717162115 – volume: 19 start-page: 287 year: 2021 ident: 9391_CR52 publication-title: Nat. Rev. Microbiol. doi: 10.1038/s41579-020-00506-3 – volume: 34 start-page: 1589 year: 2006 ident: 9391_CR38 publication-title: Crit. Care Med. doi: 10.1097/01.CCM.0000217961.75225.E9 – volume: 15 year: 2019 ident: 9391_CR48 publication-title: PLoS Comput. Biol. doi: 10.1371/journal.pcbi.1006986 – volume: 213 start-page: 1800 year: 2016 ident: 9391_CR22 publication-title: J. Infect. Dis. doi: 10.1093/infdis/jiw021 – volume: 13 start-page: 1108818 year: 2023 ident: 9391_CR25 publication-title: Front. Cell. Infect. Microbiol. doi: 10.3389/fcimb.2023.1108818 – volume: 19 start-page: 617 year: 2018 ident: 9391_CR18 publication-title: Nat. Immunol. doi: 10.1038/s41590-018-0106-2 – volume: 25 start-page: 2001779 year: 2020 ident: 9391_CR24 publication-title: Eurosurveillance doi: 10.2807/1560-7917.ES.2020.25.48.2001779 – volume: 12 start-page: 691406 year: 2021 ident: 9391_CR36 publication-title: Front. Microbiol. doi: 10.3389/fmicb.2021.691406 – volume: 14 year: 2022 ident: 9391_CR43 publication-title: mAbs doi: 10.1080/19420862.2021.2006123 – volume: 13 start-page: 1080059 year: 2022 ident: 9391_CR14 publication-title: Front. Microbiol. doi: 10.3389/fmicb.2022.1080059 – volume: 277 start-page: 25070 year: 2002 ident: 9391_CR53 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M202683200 – ident: 9391_CR3 – volume: 8 start-page: e56847 year: 2013 ident: 9391_CR41 publication-title: PLoS ONE doi: 10.1371/journal.pone.0056847 – volume: 12 year: 2020 ident: 9391_CR34 publication-title: Genome Med. doi: 10.1186/s13073-019-0706-y – volume: 10 start-page: 691 year: 2021 ident: 9391_CR33 publication-title: Antibiotics doi: 10.3390/antibiotics10060691 – volume: 10 year: 2022 ident: 9391_CR15 publication-title: Microbiol. Spectr. – volume: 152 start-page: 555 year: 2006 ident: 9391_CR40 publication-title: Microbiology doi: 10.1099/mic.0.28285-0 – volume: 167 start-page: 257 year: 1987 ident: 9391_CR54 publication-title: Carbohydr. Res. doi: 10.1016/0008-6215(87)80283-5 – volume: 108 start-page: 1304 year: 1971 ident: 9391_CR51 publication-title: J. Bacteriol. doi: 10.1128/jb.108.3.1304-1309.1971 – volume: 70 start-page: 2583 year: 2002 ident: 9391_CR39 publication-title: Infect. Immun. doi: 10.1128/IAI.70.5.2583-2590.2002 – volume: 72 start-page: 1871 year: 2021 ident: 9391_CR7 publication-title: Clin. Infect. Dis. doi: 10.1093/cid/ciaa586 – volume: 2 year: 2017 ident: 9391_CR17 publication-title: JCI Insight doi: 10.1172/jci.insight.92774 – volume: 68 start-page: 2402 year: 2000 ident: 9391_CR42 publication-title: Infect. Immun. doi: 10.1128/IAI.68.5.2402-2409.2000 – volume: 8 year: 2023 ident: 9391_CR37 publication-title: mSphere – volume: 73 start-page: 242 year: 2024 ident: 9391_CR1 publication-title: Cancer Immunol. Immunother. doi: 10.1007/s00262-024-03814-2 – volume: 3 start-page: e224 year: 2022 ident: 9391_CR8 publication-title: Lancet Microbe doi: 10.1016/S2666-5247(21)00268-8 – volume: 30 start-page: 398 year: 2024 ident: 9391_CR10 publication-title: Clin. Microbiol. Infect. doi: 10.1016/j.cmi.2023.11.005 – volume: 14 year: 2016 ident: 9391_CR29 publication-title: PLoS Biol. doi: 10.1371/journal.pbio.1002344 – volume: 8 year: 2017 ident: 9391_CR16 publication-title: Nat. Commun. doi: 10.1038/s41467-017-02223-7 – volume: 118 year: 2021 ident: 9391_CR9 publication-title: Proc. Natl. Acad. Sci. USA – volume: 101 start-page: 108305 year: 2021 ident: 9391_CR2 publication-title: Int. Immunopharmacol. doi: 10.1016/j.intimp.2021.108305 – volume: 10 start-page: 19 year: 2021 ident: 9391_CR23 publication-title: BioTech doi: 10.3390/biotech10030019 – volume: 19 year: 2023 ident: 9391_CR21 publication-title: PLoS Pathog. doi: 10.1371/journal.ppat.1011367 – volume: 26 start-page: 289 year: 2020 ident: 9391_CR35 publication-title: Emerg. Infect. Dis. doi: 10.3201/eid2602.190594 – volume: 184 start-page: 1821 year: 2021 ident: 9391_CR50 publication-title: Cell doi: 10.1016/j.cell.2021.02.035 – ident: 9391_CR49 doi: 10.1099/mgen.0.000102 – volume: 73 start-page: 001823 year: 2024 ident: 9391_CR13 publication-title: J. Med. Microbiol. doi: 10.1099/jmm.0.001823 – volume: 62 year: 2018 ident: 9391_CR30 publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.01074-18 – volume: 24 year: 2017 ident: 9391_CR20 publication-title: Clin. Vaccine Immunol. doi: 10.1128/CVI.00456-16 – volume: 49 start-page: 57 year: 2017 ident: 9391_CR31 publication-title: Infect. Chemother. doi: 10.3947/ic.2017.49.1.57 – volume: 73 start-page: 1431 year: 2021 ident: 9391_CR32 publication-title: Clin. Infect. Dis. doi: 10.1093/cid/ciab457 – volume: 9 year: 2023 ident: 9391_CR12 publication-title: Microb. Genomics doi: 10.1099/mgen.0.000967 |
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| Title | Monoclonal antibodies protect against pandrug-resistant Klebsiella pneumoniae |
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