Circulation of picobirnavirus in Neotropical free-ranging mammals

Picobirnavirus (PBV) is a family of non-enveloped double-stranded RNA viruses with bisegmented genomes. Segment 1 encodes the capsid protein and segment 2 encodes RNA-dependent RNA polymerase. They exhibit high genomic heterogeneity and infect a wide range of vertebrate hosts, including humans. The...

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Vydané v:Veterinary research communications Ročník 48; číslo 2; s. 1097 - 1109
Hlavní autori: Vanderhoeven, Ezequiel, Paresque, Roberta, Mello, Iago, Nates, Silvia, Vicentini, Fernando, Díaz, Adrián, Masachessi, Gisela
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Dordrecht Springer Netherlands 01.04.2024
Springer Nature B.V
Predmet:
Amino acids
Animals
Biomedical and Life Sciences
Capsid protein
Deer
DNA-directed RNA polymerase
Double-stranded RNA
Feces
Humans
Life Sciences
Nucleotides
Phylogenetics
Phylogeny
Picobirnavirus - genetics
RNA polymerase
RNA Virus Infections - veterinary
RNA viruses
RNA-directed RNA polymerase
Species
Veterinary Medicine/Veterinary Science
Zoology
Brazil
Atlantic Forest
Argentina
Wild ungulates
Atlantic Forest
Cerrado
Picobirnavirus
Rodents
ISSN:0165-7380, 1573-7446, 1573-7446
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Shrnutí:Picobirnavirus (PBV) is a family of non-enveloped double-stranded RNA viruses with bisegmented genomes. Segment 1 encodes the capsid protein and segment 2 encodes RNA-dependent RNA polymerase. They exhibit high genomic heterogeneity and infect a wide range of vertebrate hosts, including humans. The objective of this study was to expand our knowledge of the circulation of PBV in free-living animals from two regions (Brazil and Argentina) of the Atlantic Forest. Fecal samples were analyzed from free-living animals: tapir, brocket deer, peccary, and different species of rodents and marsupials. A total of 133 samples were collected and analyzed by RT-PCR, of which 44 (33.08%) were PBV-positive. Nine amplicons were sequenced, five species from Argentina and four from Brazil, and phylogenetic analysis was performed. The nucleotide and amino acid identities of the PBV strains detected in animals from Argentina and Brazil were between 66.3% and 82.5% and between 55.3% and 74.2%, respectively. The analysed strains presented conserved nucleotide blocks without distinction of the host species. The phylogenetic tree showed that PBV strains from Atlantic Forest animals belonging to genogroup I were grouped into different clusters, without defining groups according to host species (human or animal) or the geographical area of detection. This is the first study on PBV in free-living animals in the Atlantic Forest. Our analysis suggested that PBV strains can infect different animal species, leading to PBV transmission between animals and humans. This reinforces the hypothesis of previous crossover points in the ecology and evolution of heterologous PBV strains.
Bibliografia:ObjectType-Article-1
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ISSN:0165-7380
1573-7446
1573-7446
DOI:10.1007/s11259-023-10282-z