A widespread plant defense compound disarms bacterial type III injectisome assembly

Numerous gram-negative bacterial pathogens employ the type III secretion system (T3SS), a multiprotein injectisome, to deliver virulence proteins into host cells and cause diseases. We uncover erucamide as a previously unknown phytoalexin of both dicots and monocots that blocks the T3SS function of...

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Veröffentlicht in:Science (American Association for the Advancement of Science) Jg. 387; H. 6737; S. eads0377
Hauptverfasser: Miao, Pei, Wang, Haijun, Wang, Wei, Wang, Zhengdong, Ke, Han, Cheng, Hangyuan, Ni, Jinjing, Liang, Jingnan, Yao, Yu-Feng, Wang, Jizong, Zhou, Jian-Min, Lei, Xiaoguang
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 28.02.2025
Schlagworte:
Arabidopsis - genetics
Arabidopsis - immunology
Arabidopsis - metabolism
Arabidopsis - microbiology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Bacterial Outer Membrane Proteins - antagonists & inhibitors
Bacterial Outer Membrane Proteins - metabolism
Bacterial Proteins - metabolism
Disease Resistance
Erucic Acids - metabolism
Erucic Acids - pharmacology
Phytoalexins - metabolism
Phytoalexins - pharmacology
Plant Diseases - immunology
Plant Diseases - microbiology
Pseudomonas syringae - drug effects
Pseudomonas syringae - pathogenicity
Sesquiterpenes - chemistry
Sesquiterpenes - metabolism
Sesquiterpenes - pharmacology
Type III Secretion Systems - antagonists & inhibitors
Type III Secretion Systems - genetics
Type III Secretion Systems - metabolism
Virulence
ISSN:1095-9203, 1095-9203
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Zusammenfassung:Numerous gram-negative bacterial pathogens employ the type III secretion system (T3SS), a multiprotein injectisome, to deliver virulence proteins into host cells and cause diseases. We uncover erucamide as a previously unknown phytoalexin of both dicots and monocots that blocks the T3SS function of multiple bacterial pathogens. Genetically impairing erucamide accumulation or exogenous application in highlighted erucamide's role in antibacterial immunity. Erucamide binds Hypersensitive response and conserved C (HrcC), a key T3SS component, to block injectisome assembly. Analyses of erucamide analogs and HrcC mutants indicated that the erucamide-HrcC binding is required for inhibiting T3SS in vitro and antibacterial resistance in plants, suggesting an essential role of erucamide-HrcC binding in disease resistance. This work reveals a plant chemical defense that targets major virulence machinery in bacterial pathogens.
Bibliographie:ObjectType-Article-1
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ISSN:1095-9203
1095-9203
DOI:10.1126/science.ads0377