BSA-templated MnO2 nanoparticles as both peroxidase and oxidase mimics

Inorganic nanomaterials that mimic enzymes are fascinating as they potentially have improved properties relative to native enzymes, such as greater resistance to extremes of pH and temperature and lower sensitivity to proteases. Although many artificial enzymes have been investigated, searching for...

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Published in:Analyst (London) Vol. 137; no. 19; p. 4552
Main Authors: Liu, Xing, Wang, Qi, Zhao, Huihui, Zhang, Lichun, Su, Yingying, Lv, Yi
Format: Journal Article
Language:English
Published: England 07.10.2012
Subjects:
Animals
Antibodies - analysis
Biomimetic Materials - chemistry
Biomimetic Materials - metabolism
Catalysis
Cattle
Colorimetry
Horseradish Peroxidase - chemistry
Horseradish Peroxidase - metabolism
Humans
Hydrogen Peroxide - chemistry
Hydrogen-Ion Concentration
Immunoassay
Immunoglobulin G - immunology
Kinetics
Manganese Compounds - chemistry
Metal Nanoparticles - chemistry
Oxides - chemistry
Serum Albumin, Bovine - chemistry
Temperature
ISSN:1364-5528, 1364-5528
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Summary:Inorganic nanomaterials that mimic enzymes are fascinating as they potentially have improved properties relative to native enzymes, such as greater resistance to extremes of pH and temperature and lower sensitivity to proteases. Although many artificial enzymes have been investigated, searching for highly-efficient and stable catalysts is still of great interest. In this paper, we first demonstrated that bovine serum albumin (BSA)-stabilized MnO(2) nanoparticles (NPs) exhibited highly peroxidase-, oxidase-, and catalase-like activities. The activities of the BSA-MnO(2) NPs were evaluated using the typical horseradish peroxidase (HRP) substrates o-phenylenediamine (OPD) and 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of either hydrogen peroxide or dissolved oxygen. These small-sized BSA-MnO(2) NPs with good dispersion, solubility and biocompatibility exhibited typical Michaelis-Menten kinetics and high affinity for H(2)O(2), OPD and TMB, indicating that BSA-MnO(2) NPs can be used as satisfactory enzyme mimics. Based on these findings, BSA-MnO(2) NPs were used as colorimetric immunoassay tags for the detection of goat anti-human IgG in place of HRP. The colorimetric immunoassay using BSA-MnO(2) NPs has the advantages of being fast, robust, inexpensive, easily prepared and with no HRP and H(2)O(2) being needed. These water-soluble BSA-MnO(2) NPs may have promising potential applications in biotechnology, bioassays, and biomedicine.
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ISSN:1364-5528
1364-5528
DOI:10.1039/c2an35700c