Ameliorative Effect of Glycyrrhizic Acid on Diosbulbin B-Induced Liver Injury and Its Mechanism

This study aimed to clarify the protective effect of Glycyrrhizic acid (GL) against Diosbulbin B (DB) - induced liver injury in mice and investigate its mechanisms of action. A liver injury DB was established in mice through the oral administration of DB for 15 days. At the same time, GL was adminis...

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Published in:The American journal of Chinese medicine (1979) Vol. 53; no. 1; p. 309
Main Authors: Wang, Xin, Shi, Lei-Lei, Zhang, Yu-Han, Zhu, Hong-Zhe, Cao, Shan-Shan, Shi, Yong, Shangguan, Hui-Zi, Liu, Ji-Ping, Xie, Yun-Dong
Format: Journal Article
Language:English
Published: Singapore 2025
Subjects:
Animals
ATP-Binding Cassette Transporters - metabolism
Bile Acids and Salts - metabolism
Chemical and Drug Induced Liver Injury - drug therapy
Chemical and Drug Induced Liver Injury - etiology
Chemical and Drug Induced Liver Injury - metabolism
Disease Models, Animal
Gastrointestinal Microbiome - drug effects
Glycyrrhizic Acid - administration & dosage
Glycyrrhizic Acid - pharmacology
Glycyrrhizic Acid - therapeutic use
Heterocyclic Compounds, 4 or More Rings - adverse effects
Liver - drug effects
Liver - metabolism
Liver - pathology
Male
Mice
Oxidative Stress - drug effects
Diosbulbin B
Bile Acid
Glycyrrhizic Acid
Hepatotoxicity
Intestinal Flora
ISSN:1793-6853, 1793-6853
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Summary:This study aimed to clarify the protective effect of Glycyrrhizic acid (GL) against Diosbulbin B (DB) - induced liver injury in mice and investigate its mechanisms of action. A liver injury DB was established in mice through the oral administration of DB for 15 days. At the same time, GL was administered to the mice for treatment. After the experiment, the pharmacodynamics and mechanisms of GL in ameliorating DB-induced liver injury were explored using biochemical indexes, non-targeted metabolomics, targeted metabolomics, Western blotting analysis of protein expression, 16S rDNA sequencing, and Spearman correlation analysis. The results show reduced liver function indices and improved DB-induced hepatic pathological changes. It also attenuated DB-induced hepatic inflammation and oxidative stress. Hepatic metabolomics revealed that GL regulated ABC transporters and bile secretion. Targeted bile acid (BA) metabolomics and Western blotting demonstrated that GL improved DB-induced reduction in BA efflux by regulating FXR-mediated efflux transporters. Furthermore, analysis of 16S rDNA gene sequencing revealed that GL effectively restored the relative abundance of beneficial bacteria, reduced the relative abundance of harmful bacteria, and reinstated the structure of the intestinal flora. Additionally, correlation analyses between BA and intestinal flora indicated that , TDGA, DGA, UDGA, GDGA, THDGA, and HDGA could serve as major markers for DB-induced liver injury. In conclusion, GL significantly improved DB-induced liver injury by increasing the expression of Nrf2/FXR-BSEP/MRP2/P-gp/UGT1A1, promoting BA efflux, regulating intestinal flora, and alleviating inflammation and oxidative stress.
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ISSN:1793-6853
1793-6853
DOI:10.1142/S0192415X25500120