Development of a one-step centrifugal extraction method for simultaneous determination of multiple types of pesticides and their metabolites in urine, serum, and breast milk

•A one-step centrifugal-assisted CIPS method was developed in biological samples.•Centrifugal-assisted technique enhances performance in sample preparation.•Multiplexed tSIM method was used for highly sensitive and accurate quantification.•The greenness profile and practicality of this method were a...

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