Site-related streptococcal attachment to buccocervical tooth surfaces: A correlative micromorphologic and microbiologic study

Bevenius J, Linder L, Hultenby K. Site-related streptococcal attachment to buccocervical tooth surfaces. A correlative micromorphologx and microbiologic study. Acta Odontol Scand 1994;52:294-302. Oslo. ISSN 0001-6357. Scanning electron (SEM) microscopy of epoxy replicas made from dental impressions...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Acta odontologica Scandinavica Jg. 52; H. 5; S. 294 - 302
Hauptverfasser: Bevenius, Joan, Linder, Lars, Hultenby, Kjell
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Informa UK Ltd 1994
Taylor & Francis
Schlagworte:
Adolescent
Adult
Bacterial Adhesion - physiology
Bicuspid
Buccocervical region
Dental Enamel - microbiology
Dental Enamel - ultrastructure
Dentin - microbiology
Dentin - ultrastructure
Epoxy Resins
Female
Gingiva - microbiology
Gingiva - ultrastructure
Gingival Recession - microbiology
Gingival Recession - pathology
Gingivitis - microbiology
Gingivitis - pathology
Humans
Male
Microscopy, Electron, Scanning
Pilot Projects
replica
Replica Techniques
scanning electron microscopy
Silicones
streptococcal attachment
Streptococcus - classification
Streptococcus - isolation & purification
Streptococcus - physiology
Streptococcus mutans - isolation & purification
Streptococcus mutans - physiology
Streptococcus sobrinus - isolation & purification
Streptococcus sobrinus - physiology
Tooth - microbiology
Tooth - ultrastructure
Tooth Root - microbiology
Tooth Root - ultrastructure
ISSN:0001-6357, 1502-3850
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Bevenius J, Linder L, Hultenby K. Site-related streptococcal attachment to buccocervical tooth surfaces. A correlative micromorphologx and microbiologic study. Acta Odontol Scand 1994;52:294-302. Oslo. ISSN 0001-6357. Scanning electron (SEM) microscopy of epoxy replicas made from dental impressions has shown that in buccal gingival recession the root surfaces are devoid of cementum, leaving the dentin exposed. In this study replication techniques were applied to correlate the micro-morphology of the buccocervical region with early streptococcal attachment. The subjects were 27 healthy young adults. The buccocervical surfaces of all the premolars were meticulously cleaned. The subjects fasted for 2 h before impression-taking. Replicas were made from impressions in hydrophilic A-silicone, and streptococcal attachment was visualized by light microscopy of mitis-salivarius agar replicas incubated anaerobically for 48 h. The surface micromorphology was documented by SEM of corresponding epoxy replicas. Colonization only 2 h after cleaning was very sparse. Sites with healthy or inflamed gingivae had markedly different colonization patterns in the sulcular region. In 4 subjects with a total of 12 sites where gingival recession, undetected clinically, was disclosed by SEM, representative colonies were retrieved and identified microbiologically to species level. Two healthy sites per subject were also sampled. Streptococcus mutans and S. sobrinus were identified from eight sites with exposed root dentin. S. oralis predominated on the enamel surfaces. The method offers a valuable complement to in situ and in vitro microbiologic studies of exposed dentin and a novel technique for sampling clinical isolates of streptococci.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0001-6357
1502-3850
DOI:10.3109/00016359409029042