Mixed-Valence Pentadecavanadate with Ca2+-ATPase Inhibition Potential and Anti-Breast Cancer Activity

Polyoxovanadates are a subclass of polyoxometalates (POMs) known to interact with proteins and to present anticancer, antimicrobial, and antiviral activities. Herein, we aimed to pursue the study of the breast anticancer activity of a mixed-valence polyoxovanadate, [Cl@VV7VIV8O36]6− (V15) against MC...

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Vydané v:Inorganics Ročník 13; číslo 9; s. 306
Hlavní autori: Brito, Bianca R., Camilo, Heloísa de S., Cruz, Anderson F. da, Ribeiro, Ronny R., de Sá, Eduardo L., Camargo de Oliveira, Carolina, Fraqueza, Gil, Klassen, Giseli, Aureliano, Manuel, Nunes, Giovana G.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Basel MDPI AG 12.09.2025
Predmet:
Adenosine triphosphatase
Anticancer properties
Antitumor activity
Breast cancer
Ca2+-ATPase
Ca2+-transporting ATPase
Calcium
Calcium ions
Cancer therapies
Cell death
Cell migration
Chemotherapy
Cytotoxicity
decavanadate
Disease
Drugs
electrostatic potential map
Electrostatic properties
Enzymatic activity
Enzyme activity
Enzymes
Gene expression
Homeostasis
Melanoma
Metastasis
mixed-valence polyoxovanadates
Necroptosis
NMR
Nuclear magnetic resonance
Oxidation
P-type ATPases
Physiology
polyoxometalates
Polyoxometallates
Proteins
Signal transduction
Toxicity
Tumor cell lines
Tumors
Womens health
ISSN:2304-6740, 2304-6740
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Shrnutí:Polyoxovanadates are a subclass of polyoxometalates (POMs) known to interact with proteins and to present anticancer, antimicrobial, and antiviral activities. Herein, we aimed to pursue the study of the breast anticancer activity of a mixed-valence polyoxovanadate, [Cl@VV7VIV8O36]6− (V15) against MCF-7 and MDA-MB-231 cancer cell lines and to analyze its Ca2+-ATPase inhibition potential. 51V NMR and UV-Vis/NIR studies of V15 indicated its stability in HEPES and RPMI media. For the Ca2+-ATPase activity, V15 showed an IC50 value of 14.2 μM and a mixed type of inhibition. The electrostatic potential map of V15 and other POMs were correlated with the enzyme activity inhibition. V15 also exhibited cytotoxicity against MDA-MB-231 (IC50 = 17.2 μM) and MCF-7 (IC50 = 15.1 μM) breast cancer cell lines. Using V15 concentrations equivalent to half and 1/4 of the IC50, it was observed that MDA-MB-231 cell migration was reduced by 90 and 70%, after 24 h, respectively. Moreover, V15 caused morphological changes from fusiform to an epithelial-like (amoeboid) shape. Finally, V15 induced the increase in RIPK1, MLKL, and RIPK3 gene expression, up to 3, 10, and 15-fold, respectively, pointing out that the mechanisms of cell death in the triple-negative breast cancer cell line may occur by necroptosis.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 14
ISSN:2304-6740
2304-6740
DOI:10.3390/inorganics13090306