Analytical Method Validation and Formula Optimization of Topical Nanoemulsion Formulation Containing Resveratrol

Resveratrol (RSV), a natural lipophilic phytoalexin, was reported as an antioxidant and anti-inflammatory agent, which has the potential to cure diabetic wounds. However, several studies suggested the limitation of RSV, such as poor aqueous solubility, poor stability, and poor oral bioavailability....

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Indonesian journal of chemistry Ročník 21; číslo 5; s. 1287 - 1297
Hlavní autoři: Nastiti, Christofori Maria Ratna Rini, Riswanto, Florentinus Dika Octa
Médium: Journal Article
Jazyk:angličtina
Vydáno: Department of Chemistry, Universitas Gadjah Mada 01.10.2021
Témata:
box-behnken design
formulation
nanoemulsion
resveratrol
validation
ISSN:1411-9420, 2460-1578
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:Resveratrol (RSV), a natural lipophilic phytoalexin, was reported as an antioxidant and anti-inflammatory agent, which has the potential to cure diabetic wounds. However, several studies suggested the limitation of RSV, such as poor aqueous solubility, poor stability, and poor oral bioavailability. To overcome the issues, RSV was formulated as a topical nanoemulsion. It is important to ensure the quality of the dosage form by evaluating RSV load in the nanoformulation and optimizing the formula. A reversed-phase HPLC method was developed and validated prior to the load determination of RSV in the nanoemulsion formulation. The composition of triacetin-eugenol, Kolliphor® RH 40, and Transcutol® was further optimized by employing a Box-Behnken Design (BBD) to achieve the optimum composition with expected viscosity and RSV load. The HPLC method for determining RSV load was successfully validated for parameters of selectivity with the resolution of 8.487, linearity and range (r = 0.9979), precision (0.12% of RSD), accuracy (109–110% of recovery), the limit of detection (0.574 µg/mL), and limit of quantitation (1.740 µg/mL). The result of formula optimization was promising, showing the optimum composition of triacetin-eugenol, Kolliphor® RH 40, and Transcutol® at 4.44 g, 30.97 g, and 11.39 g, respectively.
ISSN:1411-9420
2460-1578
DOI:10.22146/ijc.63730