Enhanced microbial–substrate interactions for food waste bioconversion via biofilm-assisted anaerobic digestion: Molecular insights and profiling

This study explores the application of a biofilm-based anaerobic moving bed bioreactor (AnMBBR) to enhance microbial-substrate interactions during the anaerobic digestion (AD) of food waste (FW), representing the first use of AnMBBR for this purpose. Its performance was evaluated against a conventio...

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Vydáno v:Chemical engineering journal (Lausanne, Switzerland : 1996) Ročník 519; s. 164740
Hlavní autoři: Neeraj, Raja Ram, Nikhil, G.N.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Elsevier B.V 01.09.2025
Témata:
Fermentation
Methanogenic pathways
Microbial community dynamics
Syntrophic interactions
Waste valorization
Waste valorization
Syntrophic interactions
Fermentation
Methanogenic pathways
Microbial community dynamics
ISSN:1385-8947
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Shrnutí:This study explores the application of a biofilm-based anaerobic moving bed bioreactor (AnMBBR) to enhance microbial-substrate interactions during the anaerobic digestion (AD) of food waste (FW), representing the first use of AnMBBR for this purpose. Its performance was evaluated against a conventional anaerobic continuous stirred tank reactor (AnCSTR, control), focusing on microbial diversity and hydrolytic enzyme activity. Over 90 days (three 30-day batch cycles), AnMBBR demonstrated a higher abundance of key microbial populations, leading to increased enzyme activities and substrate solubilization peaking at 11066 ± 416 mg sCOD/L on day 10 in AnMBBR versus 10100 ± 173 mg sCOD/L on day 12 in AnCSTR. Correspondingly, peak volatile fatty acids (VFA) production was observed earlier and at higher levels in AnMBBR (5716 ± 155 mg sCOD/L on day 10) compared to AnCSTR (4736 ± 87 mg sCOD/L on day 12). The study also analyzed VFA composition and consumption, revealing higher production and conversion efficiencies in AnMBBR vs. AnCSTR: acetic acid: 2764 ± 188 mg sCOD/L (94.13 % consumed) vs. 2337 ± 44 mg sCOD/L (91.6 %); propionic acid: 709 ± 149 mg sCOD/L (93.7 %) vs. 895 ± 453 mg sCOD/L (50.94 %); butyric acid: 2377 ± 364 mg sCOD/L (100 %) vs. 1868 ± 258 mg sCOD/L (42 %). Enhanced VFA conversion in AnMBBR led to significantly higher biomethane production (165 ± 4.5 mL/g VS_added) compared to AnCSTR (88.27 ± 8.68 mL/g VS_added), attributed to the greater abundance of both acetoclastic and hydrogenotrophic methanogens. Overall, AnMBBR improved AD efficiency by enhancing microbial retention and activity. [Display omitted] •Biofilm-based reactor enhanced biogas yield compared to suspended reactor.•Hydrolytic enzyme activities improved digestion by microbial-substrate interactions.•Biofilm-based reactor showed a relatively higher abundance of methanogenic archaea.•Gene abundance, microbial community profiling and enzyme activities are co-related.
ISSN:1385-8947
DOI:10.1016/j.cej.2025.164740