A Soilborne Virus Modifies the Root System Architecture of a Plant Host via a Single Amino Acid and Influences Nematode Transmission

Grapevine fanleaf virus (GFLV) is the main causative agent of fanleaf degeneration disease. Transmission of GFLV is exclusively accomplished by the ectoparasitic dagger nematode in a noncirculative, nonpropagative mode. Previous studies of GFLV-host interactions revealed strain- and viral amino acid...

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Bibliographic Details
Published in:Phytopathology Vol. 115; no. 9; p. 1223
Main Authors: Roy, Brandon G, Demangeat, Gérard, Meyer, Sophie, Gertz, Claude, Komar, Véronique, Martin, Isabelle R, Fuchs, Marc F
Format: Journal Article
Language:English
Published: United States 01.09.2025
Subjects:
Amino Acids
Animals
Nematoda - physiology
Nematoda - virology
Nepovirus - genetics
Nepovirus - physiology
Nicotiana - parasitology
Nicotiana - virology
Plant Diseases - parasitology
Plant Diseases - virology
Plant Roots - anatomy & histology
Plant Roots - parasitology
Plant Roots - virology
Vitis - parasitology
Vitis - virology
nematode
transmission
nepovirus
virology
root system architecture
ISSN:0031-949X
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Summary:Grapevine fanleaf virus (GFLV) is the main causative agent of fanleaf degeneration disease. Transmission of GFLV is exclusively accomplished by the ectoparasitic dagger nematode in a noncirculative, nonpropagative mode. Previous studies of GFLV-host interactions revealed strain- and viral amino acid-specific changes to the root system architecture (RSA) in . Here, a two-step assay first revealed the suitability of in comparison with spp. for the transmission of GFLV by . Then, a novel one-step assay revealed strain- and viral amino acid-specific differences in transmission between wild-type GFLV strains F13 and GHu and their respective mutants, with a single residue change to position 802 of the putative RNA-dependent RNA polymerase (protein 1E ). Higher transmission rates were obtained with asymptomatic mutant GFLV-GHu 1E (33.33%, 16/48) versus symptomatic wild-type GFLV-GHu (25.53%, 12/47) and with asymptomatic wild-type GFLV-F13 (75%, 30/40) versus symptomatic mutant GFLV-F13 1E (51.28%, 20/39). These results indicate that -mediated transmission is influenced by the GFLV strain and the identity of the amino acid in position 802 of protein 1E , with a glycine favoring transmission and a lysine reducing transmission. As expected, GFLV transmission was significantly correlated with differences in RSA traits, such as the number of root tips and total root length, but not with GFLV titer in or in the roots of donor plants. This is the first report of a soilborne virus modifying both the RSA of a plant host and transmission by its dagger nematode via a single viral amino acid. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
ISSN:0031-949X
DOI:10.1094/PHYTO-03-25-0105-R