Presence of parabens in children’s faeces. Optimization and validation of a new analytical method based on the use of ultrasound-assisted extraction and liquid chromatography-tandem mass spectrometry

Due to their antimicrobial properties, parabens are a family of synthetic chemical compounds widely used as preservative additives in food and cosmetics. For this reason, humans are highly exposed to them. These substances are capable of altering the proper functioning of the endocrine system and ar...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of pharmaceutical and biomedical analysis Jg. 225; S. 115212
Hauptverfasser: Moscoso-Ruiz, Inmaculada, Navalón, Alberto, Rivas, Ana, Zafra-Gómez, Alberto
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Elsevier B.V 20.02.2023
Schlagworte:
Child
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid
Endocrine disruption
Excreta Stool
Feces - chemistry
Humans
Limit of Detection
Parabens
Parabens - analysis
Tandem Mass Spectrometry - methods
Ultra-high performance liquid chromatography-tandem mass spectrometry
Endocrine disruption
Excreta Stool
Ultra-high performance liquid chromatography-tandem mass spectrometry
Parabens
ISSN:0731-7085, 1873-264X, 1873-264X
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Due to their antimicrobial properties, parabens are a family of synthetic chemical compounds widely used as preservative additives in food and cosmetics. For this reason, humans are highly exposed to them. These substances are capable of altering the proper functioning of the endocrine system and are classified as endocrine disrupting chemicals (EDCs). Traditionally, urine has been the typical matrix studied as an excretion route. However, faeces contain valuable information. In the present study, the presence of methyl-, ethyl-, isopropyl-, propyl-, isobutyl-, butyl- and phenylparaben in stool samples from children has been evaluated. A new analytical method has been optimised and validated. The method is based on the use of ultrasound-assisted extraction followed by clean-up of the extracts by dispersive solid phase extraction dSPE). Parabens were analysed by ultrahigh performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). The matrix effect was evaluated and a significant effect was observed for all analytes. Therefore, calibration and validation were performed by addition of different concentrations of analytes to faecal blanks. The coefficient of determination (%R2) for calibration curves was higher than 98.9% in all cases. The limits of detection and quantification were between 0.2 and 0.4 and 0.6–1.0 ng g−1 respectively. The recovery for accuracy assessment had values between 89.0% and 112.7% with an RSD of less than 15% in all cases. The method was successfully applied to 14 samples from children volunteers, 100% of which showed contamination by at least one of the analysed compounds. [Display omitted] •An UHPLC-MS/MS method for the determination of parabens in human faeces is proposed.•For analyte isolation ultrasound assisted extraction is proposed.•The highest amounts found were of methylparaben, ethylparaben and propylparaben in all samples.•The method has been validated and the analytical quality parameters described.•Faeces may be considered as a good biomarker for the human exposure to parabens.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0731-7085
1873-264X
1873-264X
DOI:10.1016/j.jpba.2022.115212