The consistency and influence of environmental and animal factors on exhaled breath condensate hydrogen peroxide, pH and leukotriene B 4 in horses

This study was performed to determine the consistency of exhaled breath condensate (EBC) hydrogen peroxide (H O ), pH and leukotriene B (LTB ) measurements in asymptomatic horses and to define the influence of environmental and animal factors on these variables. Intra- and inter-day consistency for...

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Vydané v:The veterinary journal (1997) Ročník 226; s. 46 - 50
Hlavní autori: du Preez, S., Raidal, S.L., Doran, G.S., Nielsen, S.G., Hughes, K.J.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England 01.08.2017
Predmet:
Animals
Biomarkers - analysis
Environment
Exhalation
Horses
Hydrogen Peroxide - analysis
Hydrogen-Ion Concentration
Leukotriene B4 - analysis
Equine
Respiratory
EBC biomarker
Leukotriene B
HO
ISSN:1090-0233, 1532-2971
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Shrnutí:This study was performed to determine the consistency of exhaled breath condensate (EBC) hydrogen peroxide (H O ), pH and leukotriene B (LTB ) measurements in asymptomatic horses and to define the influence of environmental and animal factors on these variables. Intra- and inter-day consistency for both H O and pH measurements were adequate, with intraclass correlation coefficients ≥0.8, whereas the consistency for LTB was poor. H O was influenced by ambient temperature (T ), humidity, time of day and collection location (all P<0.01), while pH was influenced by respiratory rate during EBC collection and T (both P<0.001). The consistency of EBC H O and pH measurements may be sufficient for use as diagnostic biomarkers in horses. However, the influence of identified environmental and animal factors should be considered.
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2017.07.005