Exploring the biosynthetic potential of Korean Actinobacteria for antibacterial metabolite discovery

Actinobacteria, a phylum of Gram-positive bacteria, are renowned for their remarkable ability to produce antibacterial natural products. The National Institute of Biological Resources (NIBR) of Korea maintains a collection of Korean native actinobacteria. In this study, we explored the phylogenetic...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The journal of microbiology Jg. 63; H. 9; S. e2504002
Hauptverfasser: Park, Sehong, Je, Hyun-Woo, Cha, Yujin, Gu, Boncheol, Cho, Yeojeong, Kim, Jin-Il, Seo, Ji Won, Kim, Seung Bum, Son, Jino, Hur, Moonsuk, Sung, Changmin, Oh, Min-Kyu, Kang, Hahk-Soo
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Korea (South) 한국미생물학회 01.09.2025
Schlagworte:
Actinobacteria - classification
Actinobacteria - genetics
Actinobacteria - isolation & purification
Actinobacteria - metabolism
Anti-Bacterial Agents - biosynthesis
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - isolation & purification
Anti-Bacterial Agents - pharmacology
Biological Products - chemistry
Biological Products - isolation & purification
Biological Products - metabolism
Biological Products - pharmacology
Biosynthetic Pathways - genetics
DNA, Bacterial - genetics
Genetic Variation
Genome, Bacterial
Microbial Sensitivity Tests
Multigene Family
Phylogeny
Republic of Korea
RNA, Ribosomal, 16S - genetics
Streptomyces - genetics
Streptomyces - metabolism
생물학
Republic of Korea
actinobacteria
antibacterial activity
biosynthetic gene clusters
svetamycins
ISSN:1225-8873, 1976-3794, 1976-3794
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Actinobacteria, a phylum of Gram-positive bacteria, are renowned for their remarkable ability to produce antibacterial natural products. The National Institute of Biological Resources (NIBR) of Korea maintains a collection of Korean native actinobacteria. In this study, we explored the phylogenetic and biosynthetic diversity of the NIBR actinobacteria collection to assess its potential as a source of new antibacterial natural products. A 16S rDNA-based phylogenetic analysis revealed a high level of genetic diversity within the collection, with a predominance of Streptomyces, along with rare actinobacterial genera such as Kitasatospora and Micromonospora. Additionally, genetic network analysis of biosynthetic gene clusters (BGCs) from 15 sequenced NIBR actinobacterial strains demonstrated extensive BGC diversity, with many clusters identified as cryptic. Screening of culture extracts for antibacterial activity, followed by dereplication of active extracts, suggested the presence of potentially novel antibacterial natural products. Activity-guided isolation and whole-genome sequencing of the active strain KU57 led to the isolation of one new and three known svetamycin congeners along with their BGC. Overall, our findings highlight the NIBR actinobacteria collection as a valuable source for the discovery of new antibacterial natural products.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1225-8873
1976-3794
1976-3794
DOI:10.71150/jm.2504002