Elucidation of roles for vitamin B 12 in regulation of folate, ubiquinone, and methionine metabolism

Only a small fraction of vitamin B -requiring organisms are able to synthesize B de novo, making it a common commodity in microbial communities. Initially recognized as an enzyme cofactor of a few enzymes, recent studies have revealed additional B -binding enzymes and regulatory roles for B Here we...

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Vydané v:	Proceedings of the National Academy of Sciences - PNAS Ročník 114; číslo 7; s. E1205
Hlavní autori:	Romine, Margaret F, Rodionov, Dmitry A, Maezato, Yukari, Anderson, Lindsey N, Nandhikonda, Premchendar, Rodionova, Irina A, Carre, Alexandre, Li, Xiaoqing, Xu, Chengdong, Clauss, Therese R W, Kim, Young-Mo, Metz, Thomas O, Wright, Aaron T
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 14.02.2017
Predmet:	Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Biochemical Phenomena - radiation effects Folic Acid - metabolism Gammaproteobacteria - genetics Gammaproteobacteria - metabolism Halomonas - genetics Halomonas - metabolism Methionine - metabolism Protein Binding - radiation effects Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Ubiquinone - metabolism Ultraviolet Rays Vitamin B 12 - chemistry Vitamin B 12 - metabolism metabolism cobalamin chemical biology microbial regulation
ISSN:	1091-6490
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Shrnutí:	Only a small fraction of vitamin B -requiring organisms are able to synthesize B de novo, making it a common commodity in microbial communities. Initially recognized as an enzyme cofactor of a few enzymes, recent studies have revealed additional B -binding enzymes and regulatory roles for B Here we report the development and use of a B -based chemical probe to identify B -binding proteins in a nonphototrophic B -producing bacterium. Two unexpected discoveries resulted from this study. First, we identified a light-sensing B -binding transcriptional regulator and demonstrated that it controls folate and ubiquinone biosynthesis. Second, our probe captured proteins involved in folate, methionine, and ubiquinone metabolism, suggesting that it may play a role as an allosteric effector of these processes. These metabolic processes produce precursors for synthesis of DNA, RNA, and protein. Thereby, B likely modulates growth, and by limiting its availability to auxotrophs, B -producing organisms may facilitate coordination of community metabolism.
ISSN:	1091-6490
DOI:	10.1073/pnas.1612360114