Elucidation of roles for vitamin B 12 in regulation of folate, ubiquinone, and methionine metabolism

Only a small fraction of vitamin B -requiring organisms are able to synthesize B de novo, making it a common commodity in microbial communities. Initially recognized as an enzyme cofactor of a few enzymes, recent studies have revealed additional B -binding enzymes and regulatory roles for B Here we...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS Vol. 114; no. 7; p. E1205
Main Authors: Romine, Margaret F, Rodionov, Dmitry A, Maezato, Yukari, Anderson, Lindsey N, Nandhikonda, Premchendar, Rodionova, Irina A, Carre, Alexandre, Li, Xiaoqing, Xu, Chengdong, Clauss, Therese R W, Kim, Young-Mo, Metz, Thomas O, Wright, Aaron T
Format: Journal Article
Language:English
Published: United States 14.02.2017
Subjects:
Amino Acid Sequence
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Base Sequence
Biochemical Phenomena - radiation effects
Folic Acid - metabolism
Gammaproteobacteria - genetics
Gammaproteobacteria - metabolism
Halomonas - genetics
Halomonas - metabolism
Methionine - metabolism
Protein Binding - radiation effects
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Ubiquinone - metabolism
Ultraviolet Rays
Vitamin B 12 - chemistry
Vitamin B 12 - metabolism
metabolism
cobalamin
chemical biology
microbial regulation
ISSN:1091-6490
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Only a small fraction of vitamin B -requiring organisms are able to synthesize B de novo, making it a common commodity in microbial communities. Initially recognized as an enzyme cofactor of a few enzymes, recent studies have revealed additional B -binding enzymes and regulatory roles for B Here we report the development and use of a B -based chemical probe to identify B -binding proteins in a nonphototrophic B -producing bacterium. Two unexpected discoveries resulted from this study. First, we identified a light-sensing B -binding transcriptional regulator and demonstrated that it controls folate and ubiquinone biosynthesis. Second, our probe captured proteins involved in folate, methionine, and ubiquinone metabolism, suggesting that it may play a role as an allosteric effector of these processes. These metabolic processes produce precursors for synthesis of DNA, RNA, and protein. Thereby, B likely modulates growth, and by limiting its availability to auxotrophs, B -producing organisms may facilitate coordination of community metabolism.
ISSN:1091-6490
DOI:10.1073/pnas.1612360114