Characterization and fractionation of SARS-CoV-2 specific IgG from COVID-19 convalescent and post-vaccine group

Purpose: This study aimed to explore the method for characterization and fractionation of SARS-CoV-2 IgG specific from convalescent COVID-19 patients, post-inactive, and post-mRNA vaccines Patients and methods: The procedures include blood sample collection, SARS-CoV-2 specific IgG content screening...

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Vydáno v:Bali medical journal Ročník 14; číslo 1; s. 254 - 258
Hlavní autoři: Purwati, Nora Ertanti, Deya Karsari, Aristika Dinaryanti, Eryk Hendrianto, Igo Syaiful Ihsan, Asa Ardiana, Nanis Nurdiyati, Adita Ayu Permanasari, Bagus Satrio Nurwito, Andang Miatmoko
Médium: Journal Article
Jazyk:angličtina
Vydáno: 01.01.2025
ISSN:2089-1180, 2302-2914
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Abstract Purpose: This study aimed to explore the method for characterization and fractionation of SARS-CoV-2 IgG specific from convalescent COVID-19 patients, post-inactive, and post-mRNA vaccines Patients and methods: The procedures include blood sample collection, SARS-CoV-2 specific IgG content screening, IgG fractionation, and validate specific IgG. Statistical analysis was carried out to evaluate the significance value of SARS-CoV-2 IgG presence in each sample group. Results: The results showed that the post-mRNA vaccine samples had the highest levels of SARS-CoV-2 IgG antibodies. Furthermore, the validation was confirmed to be specific to SARS-CoV-2 IgG, as indicated by the heavy and light chains marked at ~50kDa and 25kDa, respectively. Conclusion: A significant difference between the SARS-CoV-2 IgG content between the three sample groups is indicated by a P value of 0.0043.
AbstractList Purpose: This study aimed to explore the method for characterization and fractionation of SARS-CoV-2 IgG specific from convalescent COVID-19 patients, post-inactive, and post-mRNA vaccines Patients and methods: The procedures include blood sample collection, SARS-CoV-2 specific IgG content screening, IgG fractionation, and validate specific IgG. Statistical analysis was carried out to evaluate the significance value of SARS-CoV-2 IgG presence in each sample group. Results: The results showed that the post-mRNA vaccine samples had the highest levels of SARS-CoV-2 IgG antibodies. Furthermore, the validation was confirmed to be specific to SARS-CoV-2 IgG, as indicated by the heavy and light chains marked at ~50kDa and 25kDa, respectively. Conclusion: A significant difference between the SARS-CoV-2 IgG content between the three sample groups is indicated by a P value of 0.0043.
Author Deya Karsari
Nora Ertanti
Aristika Dinaryanti
Eryk Hendrianto
Igo Syaiful Ihsan
Nanis Nurdiyati
Adita Ayu Permanasari
Bagus Satrio Nurwito
Andang Miatmoko
Purwati
Asa Ardiana
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