Effect of Different Melanin-bleaching Methods on Staining
The melanin affects the pathologic diagnosis, so melanin bleaching must be performed. Our study was to investigate the effect of different melanin-bleaching methods on hematoxylin-eosin staining and immunohistochemical staining. We were used 10 malignant melanoma tissues as experimental subjects, an...
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| Vydáno v: | Applied immunohistochemistry & molecular morphology |
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| Hlavní autoři: | , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
United States
03.11.2025
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| Témata: | |
| ISSN: | 1533-4058, 1533-4058 |
| On-line přístup: | Zjistit podrobnosti o přístupu |
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| Shrnutí: | The melanin affects the pathologic diagnosis, so melanin bleaching must be performed. Our study was to investigate the effect of different melanin-bleaching methods on hematoxylin-eosin staining and immunohistochemical staining. We were used 10 malignant melanoma tissues as experimental subjects, and 3 tissues from T cell lymphoma, B cell lymphoma, neurofibroma and colon as control. Melanin was detected in all 10 malignant melanoma tissues before bleaching. Our study compared among permanganate/oxalic acid treatment, hydrogen peroxide treatment, and double oxidation treatment. The antibodies CD3 and MUM1 negative after potassium permanganate/oxalic acid treatment, but normal expression after hydrogen peroxide treatment. Antibodies CD20 and S-100 can be expressed normally after bleaching. Thus, the 10% hydrogen peroxide for 24 hours at 37°C is the mildest on melanin bleaching, its almost no effect on hematoxylin-eosin staining and immunohistochemical staining. It is suitable for different tissues and antibodies. |
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| Bibliografie: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1533-4058 1533-4058 |
| DOI: | 10.1097/PAI.0000000000001291 |