Short-term outcomes of corneal epithelial reconstruction in patients with bilateral limbal stem cell deficiency using paralimbal oral mucosal epithelial transplantation

This study analyzed the outcomes of corneal epithelium reconstruction using the method of paralimbal oral mucosal epithelial transplantation (PLOMET) in patients with bilateral limbal stem cell deficiency. PLOMET surgery was performed in 10 patients with bilateral limbal stem cell deficiency. Surgic...

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Vydané v:Vestnik oftal'mologii Ročník 141; číslo 5; s. 54
Hlavní autori: Isabekov, R S, Gelyastanov, A M, Malyugin, B E, Borzenok, S A, Ostrovskiy, D S, Izmaylova, S B
Médium: Journal Article
Jazyk:English
Russian
Vydavateľské údaje: Russia (Federation) 2025
Predmet:
Adult
Corneal Diseases - diagnosis
Corneal Diseases - physiopathology
Corneal Diseases - surgery
Epithelium, Corneal - pathology
Epithelium, Corneal - surgery
Female
Humans
Limbal Stem Cell Deficiency
Limbus Corneae - pathology
Limbus Corneae - surgery
Male
Middle Aged
Mouth Mucosa - cytology
Mouth Mucosa - transplantation
Re-Epithelialization
Stem Cell Transplantation - methods
Stem Cells - pathology
Treatment Outcome
Visual Acuity
limbal stem cell deficiency
bilateral limbal stem cell deficiency
oral mucosal epithelial transplantation
corneal burn
ISSN:0042-465X
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Popis
Shrnutí:This study analyzed the outcomes of corneal epithelium reconstruction using the method of paralimbal oral mucosal epithelial transplantation (PLOMET) in patients with bilateral limbal stem cell deficiency. PLOMET surgery was performed in 10 patients with bilateral limbal stem cell deficiency. Surgical outcomes were monitored for 12 months postoperatively. Impression cytology of the ocular surface with immunohistochemical (IHC) analysis for specific keratins (K3, K4, K7, K12) was performed before and after surgery in addition to standard and supplementary diagnostic methods. Corneal re-epithelialization within 1-2 months was achieved in all patients. In the late postoperative period, 2 out of 10 patients experienced spontaneous epithelial erosions, associated with systemic comorbidities and certain environmental factors. At 12 months postoperatively, 9 out of 10 patients demonstrated slight improvement in visual acuity, and best-corrected visual acuity exceeded 0.1 in 2 out of 10 patients. Grading of corneal status by three independent experts demonstrated a reduction in conjunctivalization, decreased neovascularization, and improved corneal transparency in 8 out of 10 patients. IHC results showed positive staining for K4, characteristic of oral mucosal epithelial cells, in 7 out of 8 patients, whereas only one patient exhibited marked K12 expression characteristic of corneal epithelium. Paralimbal oral mucosa epithelial transplantation (PLOMET) enables stable corneal re-epithelialization and partial improvement in visual acuity. The latter is limited by the degree of corneal stromal opacification, providing a rationale for optical keratoplasty as a second stage of visual rehabilitation in this patient population.
Bibliografia:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0042-465X
DOI:10.17116/oftalma202514105154