Laboratory diagnosis of Neisseria gonorrhoeae in St Petersburg, Russia: inventory, performance characteristics and recommended optimisations

Objectives: To perform a comprehensive inventory of the number of samples, performance characteristics, and quality assurance of the laboratory diagnosis of Neisseria gonorrhoeae at five laboratories in St Petersburg and Leningradskaya Oblast, Russia, in 2004, and to recommend optimisations for an i...

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Bibliographic Details
Published in:Sexually transmitted infections Vol. 82; no. 1; pp. 41 - 44
Main Authors: Unemo, M, Savicheva, A, Budilovskaya, O, Sokolovsky, E, Larsson, M, Domeika, M
Format: Journal Article
Language:English
Published: London BMJ 01.02.2006
BMJ Publishing Group LTD
BMJ Group
Subjects:
Cervix
Clinical Laboratory Techniques - standards
Clinical Laboratory Techniques - statistics & numerical data
Diagnostics
Female
Gonorrhea
Gonorrhea - diagnosis
Humans
Laboratories
Laboratory Techniques and Procedures/standards/statistics & numerical data
Male
MEDICIN
MEDICINE
Microbial Sensitivity Tests - standards
Microbial Sensitivity Tests - statistics & numerical data
Microbial Sensitivity Tests/standards/statistics & numerical data
Microbiology, immunology, infectious diseases
Microscopy
Mikrobiologi, immunologi, infektionssjukdomar
Neisseria gonorrhoeae
Neisseria gonorrhoeae - growth & development
Quality Assurance, Health Care
Quality control
Questionnaires
Rectum
Russia
Sensitivity and Specificity
Russia
Research Support
Health Care
Quality Assurance
Non-U.S. Gov't
ISSN:1368-4973, 1472-3263, 1472-3263
Online Access:Get full text
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Summary:Objectives: To perform a comprehensive inventory of the number of samples, performance characteristics, and quality assurance of the laboratory diagnosis of Neisseria gonorrhoeae at five laboratories in St Petersburg and Leningradskaya Oblast, Russia, in 2004, and to recommend optimisations for an increased adherence to international evidence based recommendations of diagnostics. Methods: Surveillance data were obtained with questionnaire and site visits. For evaluation of the culture media utilised at the laboratories, N gonorrhoeae reference strains (n = 29) were used. Results: During 2004 the total numbers of N gonorrhoeae samples analysed at the five laboratories using microscopy of stained smears and culturing were 330 879 (407 positive) and 38 020 (420 positive), respectively. Four laboratories used a Russian non-selective culture medium—that is, Complegon, and one laboratory utilised Biocult-GC. Both media seemed suboptimal. Only two of the laboratories used any species confirmative assay. Antibiotic susceptibility testing of N gonorrhoeae was performed at only two of the laboratories and each year only occasional isolates were analysed. None of the laboratories comprised a complete laboratory quality assurance system. Conclusions: According to international recommendations, the diagnosis of N gonorrhoeae in St Petersburg and Leningradskaya Oblast, Russia, is suboptimal. More samples need to be analysed by culturing on a highly nutritious and selective medium and, furthermore, species confirmation and antibiotic susceptibility testing should be more frequently performed. In addition, the utilised methods for culturing and antibiotic susceptibility testing, including medium and interpretative criteria used, ought to be optimised, standardised, and quality assured using systematic internal and external quality controls.
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ISSN:1368-4973
1472-3263
1472-3263
DOI:10.1136/sti.2005.016345