Biochemical properties, enterohaemolysin production and plasmid carriage of Shiga toxin-producing Escherichia coli strains

Thirty-eight strains of Shiga toxin-producing Escherichia coli (STEC) were characterised in terms of biochemical properties, enterohaemolysin production and plasmid carriage. A wide variation in the biochemical properties was observed among the STEC, with 14 distinct biotypes identified. Biotype 1 w...

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Published in:Memórias do Instituto Oswaldo Cruz Vol. 105; no. 3; pp. 318 - 321
Main Authors: Souza, Mario R.S.R.S.M, Klassen, Giseli, De Toni, Fabiana, Rigo, Liu U, Henkes, Caroline, Pigatto, Caroline P, Dalagassa, Cibelle de Borba, Fadel-Picheth, Cyntia M.T
Format: Journal Article
Language:English
Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01.05.2010
Instituto Oswaldo Cruz, Ministério da Saúde
Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:
Animals
biochemical properties
Cattle
Child
DNA, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field
enterohaemolysin
Escherichia coli
Escherichia coli Proteins - biosynthesis
Escherichia coli, STEC, plasmid profile, enterohaemolysin, biochemical properties
Hemolysin Proteins - biosynthesis
Humans
PARASITOLOGY
plasmid profile
Plasmids - genetics
Shiga-Toxigenic Escherichia coli - genetics
Shiga-Toxigenic Escherichia coli - metabolism
STEC
TROPICAL MEDICINE
STEC
biochemical properties
plasmid profile
enterohaemolysin
Escherichia coli
ISSN:1678-8060, 0074-0276, 0074-0276, 1678-8060
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Summary:Thirty-eight strains of Shiga toxin-producing Escherichia coli (STEC) were characterised in terms of biochemical properties, enterohaemolysin production and plasmid carriage. A wide variation in the biochemical properties was observed among the STEC, with 14 distinct biotypes identified. Biotype 1 was the most common, found in 29% of the strains. Enterohaemolysin production was detected in 29% of the strains. Most of the bacterial strains (95%) carried one or more plasmids and considerable heterogeneity in size and combinations was observed. Seven distinct plasmid profiles were identified. The most common profile, characterised by the presence of a single plasmid of ~90 kb, was found in 50% of these strains. These data indicate extensive diversity among STEC strains. No correlation was found among biotype, serotype, enterohaemolysin production and plasmid profile.
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ISSN:1678-8060
0074-0276
0074-0276
1678-8060
DOI:10.1590/S0074-02762010000300013