The stress kit: a new method based on competitive reverse transcriptase–polymerase chain reaction to quantify the expression of human αB-crystallin, Hsp27, and Hsp60

We describe a reverse transcriptase–polymerase chain reaction method for the semiquantitative detection of mRNAs encoding the human heat shock proteins αB-crystallin, Hsp27, and Hsp60. The method involves the coamplification of cellular mRNA-derived cDNA with a dilution series of a competitor fragme...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Cell stress & chaperones Jg. 5; H. 1; S. 30 - 35
Hauptverfasser:	Bajramović, Jeffrey J., Geutskens, Sacha B., Bsibsi, Malika, Boot, Marit, Hassankhan, Ryan, Verhulst, Karien C., van Noort, Johannes M.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Cell Stress Society International 01.01.2000
Schlagworte:	Cell lines Complementary DNA Electrophoresis Gels Heat shock proteins Messenger RNA Neuroglia Polymerase chain reaction Regular s Reverse transcriptase polymerase chain reaction Shock heating
ISSN:	1355-8145, 1466-1268
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!