Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study

The molecular mechanism formation of secondary epiretinal membranes (ERMs) after proliferative diabetic retinopathy (PDR) or primary idiopathic ERMs is still poorly understood. Therefore, the present study focused on the assessment of IGF1, IGF1R, and IGFBP3 mRNA levels in ERMs and PBMCs from patien...

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Veröffentlicht in:	Mediators of Inflammation Jg. 2013; H. 2013; S. 662 - 668
Hauptverfasser:	Kimsa, Malgorzata W., Romaniuk, Dorota, Strzalka-Mrozik, Barbara, Kimsa, Magdalena C., Kabiesz, Adam, Romaniuk, Wanda, Mazurek, Urszula
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Cairo, Egypt Hindawi Limiteds 01.01.2013 Hindawi Puplishing Corporation Hindawi Publishing Corporation John Wiley & Sons, Inc Wiley
Schlagworte:	Aged Aged, 80 and over Clinical Study Development and progression Diabetic nephropathies Diabetic Retinopathy - etiology Diabetic Retinopathy - metabolism Diabetic Retinopathy - surgery Epiretinal Membrane - metabolism Female Genetic aspects Growth factors Humans Insulin-Like Growth Factor Binding Protein 3 - genetics Insulin-Like Growth Factor Binding Protein 3 - physiology Insulin-Like Growth Factor I - genetics Insulin-Like Growth Factor I - physiology Leukocytes, Mononuclear - metabolism Male Messenger RNA Properties Real-Time Polymerase Chain Reaction Receptor, IGF Type 1 - genetics Receptor, IGF Type 1 - physiology Reverse Transcriptase Polymerase Chain Reaction Vitreoretinopathy, Proliferative - etiology
ISSN:	0962-9351, 1466-1861, 1466-1861
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Zusammenfassung:	The molecular mechanism formation of secondary epiretinal membranes (ERMs) after proliferative diabetic retinopathy (PDR) or primary idiopathic ERMs is still poorly understood. Therefore, the present study focused on the assessment of IGF1, IGF1R, and IGFBP3 mRNA levels in ERMs and PBMCs from patients with PDR. The examined group comprised 6 patients with secondary ERMs after PDR and the control group consisted of 11 patients with idiopathic ERMs. Quantification of IGF1, IGF1R, and IGFBP3 mRNAs was performed by real-time QRT-PCR technique. In ERMs, IGF1 and IGF1R mRNA levels were significantly higher in patients with diabetes compared to control subjects. In PBMCs, there were no statistically significant differences of IGF1, IGF1R, and IGFBP3 expression between diabetic and nondiabetic patients. In conclusion, our study indicated IGF1 and IGF1R differential expression in ERMs, but not in PBMCs, of diabetic and nondiabetic patients, suggesting that these factors can be involved in the pathogenesis or progression of proliferative vitreoretinal disorders. This trial is registered with NCT00841334.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Academic Editor: Dorota Raczynska
ISSN:	0962-9351 1466-1861 1466-1861
DOI:	10.1155/2013/986217