Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing

In 16S rRNA gene sequencing, certain bacterial genera were found to be underrepresented or even missing in taxonomic profiles when using unsuitable primer combinations, outdated reference databases, or inadequate pipeline settings. Concerning the last, quality thresholds as well as bioinformatic set...

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Vydané v:	mSphere Ročník 6; číslo 1
Hlavní autori:	Abellan-Schneyder, Isabel, Matchado, Monica S., Reitmeier, Sandra, Sommer, Alina, Sewald, Zeno, Baumbach, Jan, List, Markus, Neuhaus, Klaus
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States American Society for Microbiology 24.02.2021
Predmet:	Research Article databases variable regions bioinformatic settings mock communities 16S rRNA gene sequencing amplicon sequencing clustering microbiome
ISSN:	2379-5042, 2379-5042
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