Nuclease-Resistant DNA via High-Density Packing in Polymeric Micellar Nanoparticle Coronas

Herein, we describe a polymeric micellar nanoparticle capable of rendering nucleic acids resistant to nuclease digestion. This approach relies on utilizing DNA as the polar headgroup of a DNA–polymer amphiphile in order to assemble well-defined, discrete nanoparticles. Dense packing of DNA in the mi...

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Bibliographic Details
Published in:ACS nano Vol. 7; no. 2; pp. 1379 - 1387
Main Authors: Rush, Anthony M, Thompson, Matthew P, Tatro, Erick T, Gianneschi, Nathan C
Format: Journal Article
Language:English
Published: United States American Chemical Society 26.02.2013
Subjects:
Base Sequence
Coronas
Deoxyribonucleases - metabolism
Deoxyribonucleic acid
Digestion
DNA - chemistry
DNA - genetics
DNA - metabolism
Enzymes
Hydrophobic and Hydrophilic Interactions
Micelles
Models, Molecular
Nanoparticles
Nanoparticles - chemistry
Nanostructure
Nucleic Acid Conformation
Oligonucleotides
Polymers - chemistry
Purification
Rendering
polymer
nanotechnology
nuclease
DNA
resistance
ISSN:1936-0851, 1936-086X, 1936-086X
Online Access:Get full text
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Summary:Herein, we describe a polymeric micellar nanoparticle capable of rendering nucleic acids resistant to nuclease digestion. This approach relies on utilizing DNA as the polar headgroup of a DNA–polymer amphiphile in order to assemble well-defined, discrete nanoparticles. Dense packing of DNA in the micelle corona allows for hybridization of complementary oligonucleotides while prohibiting enzymatic degradation. We demonstrate the preparation, purification, and characterization of the nanoparticles, then describe their resistance to treatment with endo- and exonucleases including snake-venom phosphodiesterase (SVP), a common, general DNA digestion enzyme.
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ISSN:1936-0851
1936-086X
1936-086X
DOI:10.1021/nn305030g