Nuclease-Resistant DNA via High-Density Packing in Polymeric Micellar Nanoparticle Coronas

Herein, we describe a polymeric micellar nanoparticle capable of rendering nucleic acids resistant to nuclease digestion. This approach relies on utilizing DNA as the polar headgroup of a DNA–polymer amphiphile in order to assemble well-defined, discrete nanoparticles. Dense packing of DNA in the mi...

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Vydáno v:	ACS nano Ročník 7; číslo 2; s. 1379 - 1387
Hlavní autoři:	Rush, Anthony M, Thompson, Matthew P, Tatro, Erick T, Gianneschi, Nathan C
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States American Chemical Society 26.02.2013
Témata:	Base Sequence Coronas Deoxyribonucleases - metabolism Deoxyribonucleic acid Digestion DNA - chemistry DNA - genetics DNA - metabolism Enzymes Hydrophobic and Hydrophilic Interactions Micelles Models, Molecular Nanoparticles Nanoparticles - chemistry Nanostructure Nucleic Acid Conformation Oligonucleotides Polymers - chemistry Purification Rendering polymer nanotechnology nuclease DNA resistance
ISSN:	1936-0851, 1936-086X, 1936-086X
On-line přístup:	Získat plný text
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Shrnutí:	Herein, we describe a polymeric micellar nanoparticle capable of rendering nucleic acids resistant to nuclease digestion. This approach relies on utilizing DNA as the polar headgroup of a DNA–polymer amphiphile in order to assemble well-defined, discrete nanoparticles. Dense packing of DNA in the micelle corona allows for hybridization of complementary oligonucleotides while prohibiting enzymatic degradation. We demonstrate the preparation, purification, and characterization of the nanoparticles, then describe their resistance to treatment with endo- and exonucleases including snake-venom phosphodiesterase (SVP), a common, general DNA digestion enzyme.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1936-0851 1936-086X 1936-086X
DOI:	10.1021/nn305030g