BioPlex Display: An Interactive Suite for Large-Scale AP-MS Protein-Protein Interaction Data

The development of large-scale data sets requires a new means to display and disseminate research studies to large audiences. Knowledge of protein-protein interaction (PPI) networks has become a principle interest of many groups within the field of proteomics. At the confluence of technologies, such...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Journal of proteome research Ročník 17; číslo 1; s. 722
Hlavní autoři: Schweppe, Devin K, Huttlin, Edward L, Harper, J Wade, Gygi, Steven P
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 05.01.2018
Témata:
Computational Biology - methods
Databases, Protein
Humans
Mass Spectrometry
Protein Interaction Mapping - methods
Protein Interaction Maps
protein interactions
AP−MS
graph display
web application
term enrichment
network communities
PHP/MySQL
data visualization
protein complexes
BioPlex
ISSN:1535-3907, 1535-3907
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:The development of large-scale data sets requires a new means to display and disseminate research studies to large audiences. Knowledge of protein-protein interaction (PPI) networks has become a principle interest of many groups within the field of proteomics. At the confluence of technologies, such as cross-linking mass spectrometry, yeast two-hybrid, protein cofractionation, and affinity purification mass spectrometry (AP-MS), detection of PPIs can uncover novel biological inferences at a high-throughput. Thus new platforms to provide community access to large data sets are necessary. To this end, we have developed a web application that enables exploration and dissemination of the growing BioPlex interaction network. BioPlex is a large-scale interactome data set based on AP-MS of baits from the human ORFeome. The latest BioPlex data set release (BioPlex 2.0) contains 56 553 interactions from 5891 AP-MS experiments. To improve community access to this vast compendium of interactions, we developed BioPlex Display, which integrates individual protein querying, access to empirical data, and on-the-fly annotation of networks within an easy-to-use and mobile web application. BioPlex Display enables rapid acquisition of data from BioPlex and development of hypotheses based on protein interactions.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1535-3907
1535-3907
DOI:10.1021/acs.jproteome.7b00572