A Mitochondria-Targeted Cryptocyanine-Based Photothermogenic Photosensitizer

Cryptocyanine-based probes exhibit highly efficient photothermal conversion and represent a new class of photothermal agents for use in photothermal therapy (PTT). With the thermal susceptibility of mitochondria in mind, we have prepared a mitochondria targeted, NIR-absorbing cryptocyanine probe (Mi...

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Veröffentlicht in:Journal of the American Chemical Society Jg. 139; H. 29; S. 9972 - 9978
Hauptverfasser: Jung, Hyo Sung, Lee, Jae-Hong, Kim, Kyutae, Koo, Seyoung, Verwilst, Peter, Sessler, Jonathan L., Kang, Chulhun, Kim, Jong Seung
Format: Journal Article
Sprache:Englisch
Veröffentlicht: WASHINGTON Amer Chemical Soc 26.07.2017
Schlagworte:
Carbocyanines - chemistry
Carbocyanines - pharmacology
Chemistry
Chemistry, Multidisciplinary
HeLa Cells
Humans
Infrared Rays
Mitochondria - drug effects
Molecular Structure
Photochemotherapy
Photosensitizing Agents - chemistry
Photosensitizing Agents - pharmacology
Physical Sciences
Science & Technology
Temperature
THERAPEUTICS
HYPERTHERMIA
CELLS
DYE
NANOPARTICLES
PHOTODYNAMIC THERAPY
ABLATION
ABSORPTION
CANCER-THERAPY
ISSN:0002-7863, 1520-5126, 1520-5126
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Zusammenfassung:Cryptocyanine-based probes exhibit highly efficient photothermal conversion and represent a new class of photothermal agents for use in photothermal therapy (PTT). With the thermal susceptibility of mitochondria in mind, we have prepared a mitochondria targeted, NIR-absorbing cryptocyanine probe (Mito-CCy) and evaluated its photophysical properties, photothermal conversion efficiency, biological compatibility, cytotoxicity, and mitochondrial localization in HeLa cells. Upon subjecting 0.5 mL of a PBS buffer solution (10 mM, pH 7.4, containing 50% DMSO) of Mito-CCy (0.5 mM) to 730 nm laser irradiation at 2.3 W/cm(2), the temperature of the solution increased by 13.5 degrees C within 5 min. In contrast, the corresponding cryptocyanine (CCy) lacking the triarylphosphonium group gave rise to only an similar to 3.4 degrees C increase in solution temperature under otherwise identical conditions. Mito-CCy also exhibited high cytotoxicity in HeLa cells when subject to photoirradiation. This light-induced cytotoxicity is, attributed to the endogenous production of reactive oxygen species (ROS) induced under conditions, of local heating. ROS are known to interfere with the mitochondrial defense system and to trigger apoptosis. By targeting the mitochondria, the present sensitizer-based photothermogenic approach is rendered more effective. As such, the system reported here represents the vanguard of what might be a new generation of organelle-targeted photothermal therapeutics.
Bibliographie:NIH RePORTER
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-7863
1520-5126
1520-5126
DOI:10.1021/jacs.7b04263