High-Throughput Glycomic Methods

Glycomics aims to identify the structure and function of the glycome, the complete set of oligosaccharides (glycans), produced in a given cell or organism, as well as to identify genes and other factors that govern glycosylation. This challenging endeavor requires highly robust, sensitive, and poten...

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Bibliographic Details
Published in:Chemical reviews Vol. 122; no. 20; p. 15865
Main Authors: Trbojević-Akmačić, Irena, Lageveen-Kammeijer, Guinevere S M, Heijs, Bram, Petrović, Tea, Deriš, Helena, Wuhrer, Manfred, Lauc, Gordan
Format: Journal Article
Language:English
Published: United States 26.10.2022
Subjects:
Glycomics - methods
Glycosylation
Polysaccharides - chemistry
ISSN:1520-6890, 1520-6890
Online Access:Get more information
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Summary:Glycomics aims to identify the structure and function of the glycome, the complete set of oligosaccharides (glycans), produced in a given cell or organism, as well as to identify genes and other factors that govern glycosylation. This challenging endeavor requires highly robust, sensitive, and potentially automatable analytical technologies for the analysis of hundreds or thousands of glycomes in a timely manner (termed high-throughput glycomics). This review provides a historic overview as well as highlights recent developments and challenges of glycomic profiling by the most prominent high-throughput glycomic approaches, with -glycosylation analysis as the focal point. It describes the current state-of-the-art regarding levels of characterization and most widely used technologies, selected applications of high-throughput glycomics in deciphering glycosylation process in healthy and disease states, as well as future perspectives.
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ISSN:1520-6890
1520-6890
DOI:10.1021/acs.chemrev.1c01031